Journal article
Topologically correct central projections of tetrapod inner ear afferents require Fzd3
Scientific reports, Vol.9(1), pp.10298-14
07/16/2019
DOI: 10.1038/s41598-019-46553-6
PMCID: PMC6635624
PMID: 31311957
Abstract
Inner ear sensory afferent connections establish sensory maps between the inner ear hair cells and the vestibular and auditory nuclei to allow vestibular and sound information processing. While molecular guidance of sensory afferents to the periphery has been well studied, molecular guidance of central projections from the ear is only beginning to emerge. Disorganized central projections of spiral ganglion neurons in a Wnt/PCP pathway mutant,
Prickle1
, suggest the Wnt/PCP pathway plays a role in guiding cochlear afferents to the cochlear nuclei in the hindbrain, consistent with known expression of the Wnt receptor,
Frizzled3
(
Fzd3
) in inner ear neurons. We therefore investigated the role of Wnt signaling in central pathfinding in
Fzd3
mutant mice and
Fzd3
morpholino treated frogs and found aberrant central projections of vestibular afferents in both cases. Ear transplantations from knockdown to control
Xenopus
showed that it is the
Fzd3
expressed within the ear that mediates this guidance. Also, cochlear afferents of
Fzd3
mutant mice lack the orderly topological organization observed in controls. Quantification of
Fzd3
expression in spiral ganglion neurons show a gradient of expression with
Fzd3
being higher in the apex than in the base. Together, these results suggest that a gradient of
Fzd3
in inner ear afferents directs projections to the correct dorsoventral column within the hindbrain.
Details
- Title: Subtitle
- Topologically correct central projections of tetrapod inner ear afferents require Fzd3
- Creators
- Jeremy S Duncan - Kalamazoo, MI USABernd Fritzsch - Iowa City, IA USADouglas W Houston - Iowa City, IA USAElizabeth M Ketchum - Kalamazoo, MI USAJennifer Kersigo - Iowa City, IA USAMichael R Deans - Salt Lake City, UT USAKaren L Elliott - Iowa City, IA USA
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.9(1), pp.10298-14
- DOI
- 10.1038/s41598-019-46553-6
- PMID
- 31311957
- PMCID
- PMC6635624
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Publisher
- Nature Publishing Group UK; London
- Grant note
- DC017589; DC013066; DC015333 / ; AG060504 / ;
- Language
- English
- Date published
- 07/16/2019
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070716602771
Metrics
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