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Total sulfane sulfur bioavailability reflects ethnic and gender disparities in cardiovascular disease
Journal article   Open access   Peer reviewed

Total sulfane sulfur bioavailability reflects ethnic and gender disparities in cardiovascular disease

Saurabh Rajpal, Pavan Katikaneni, Matthew Deshotels, Sibile Pardue, John Glawe, Xinggui Shen, Nuri Akkus, Kalgi Modi, Ruchi Bhandari, Paari Dominic, …
Redox biology, Vol.15(C), pp.480-489
05/2018
DOI: 10.1016/j.redox.2018.01.007
PMCID: PMC5975076
PMID: 29413960
url
https://doi.org/10.1016/j.redox.2018.01.007View
Published (Version of record) Open Access

Abstract

Hydrogen sulfide (H2S) has emerged as an important physiological and pathophysiological signaling molecule in the cardiovascular system influencing vascular tone, cytoprotective responses, redox reactions, vascular adaptation, and mitochondrial respiration. However, bioavailable levels of H2S in its various biochemical metabolite forms during clinical cardiovascular disease remain poorly understood. We performed a case-controlled study to quantify and compare the bioavailability of various biochemical forms of H2S in patients with and without cardiovascular disease (CVD). In our study, we used the reverse-phase high performance liquid chromatography monobromobimane assay to analytically measure bioavailable pools of H2S. Single nucleotide polymorphisms (SNPs) were also identified using DNA Pyrosequencing. We found that plasma acid labile sulfide levels were significantly reduced in Caucasian females with CVD compared with those without the disease. Conversely, plasma bound sulfane sulfur levels were significantly reduced in Caucasian males with CVD compared with those without the disease. Surprisingly, gender differences of H2S bioavailability were not observed in African Americans, although H2S bioavailability was significantly lower overall in this ethnic group compared to Caucasians. We also performed SNP analysis of H2S synthesizing enzymes and found a significant increase in cystathionine gamma-lyase (CTH) 1364 G-T allele frequency in patients with CVD compared to controls. Lastly, plasma H2S bioavailability was found to be predictive for cardiovascular disease in Caucasian subjects as determined by receiver operator characteristic analysis. These findings reveal that plasma H2S bioavailability could be considered a biomarker for CVD in an ethnic and gender manner. Cystathionine gamma-lyase 1346 G-T SNP might also contribute to the risk of cardiovascular disease development. •Baseline plasma sulfide metabolite levels are significantly different in an ethnic dependent manner.•Reductions in sulfide metabolites are predictive of cardiovascular disease in an ethnic dependent manner.•Differences in acid labile versus bound sulfane sulfur metabolites during cardiovascular disease are gender dependent.•Single nucleotide polymorphism of CTH 1364 G>T is significantly associated with increased cardiovascular disease.
 Coronary artery disease HPLC Hydrogen sulfide Monobromobimane Peripheral artery disease

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