Journal article
Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System
Journal of bacteriology, Vol.182(3), pp.842-847
Note
02/2000
DOI: 10.1128/jb.182.3.842-847.2000
PMCID: PMC94354
PMID: 10633125
Abstract
We describe a simple system for reversible, stable integration of plasmid-borne genes into the
Escherichia coli
chromosome. Most ordinary
E. coli
strains and a variety of pBR322-derived ampicillin-resistant plasmids can be used. A single genetic element, a lambda phage, is the only specialized vector required. The resultant strains have a single copy of the plasmid fragment inserted stably at the lambda attachment site on the chromosome, with nearly the entire lambda genome deleted.
Details
- Title: Subtitle
- Towards Single-Copy Gene Expression Systems Making Gene Cloning Physiologically Relevant: Lambda InCh, a Simple Escherichia coli Plasmid-Chromosome Shuttle System
- Creators
- Dana Boyd - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115David S Weiss - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115Joseph C Chen - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115Jon Beckwith - Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.182(3), pp.842-847
- Series
- Note
- DOI
- 10.1128/jb.182.3.842-847.2000
- PMID
- 10633125
- PMCID
- PMC94354
- NLM abbreviation
- J Bacteriol
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 02/2000
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984002385202771
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