Journal article
Toxic Shock Syndrome Toxin 1 Is Encoded by a Variable Genetic Element
Clinical infectious diseases, Vol.11(Supplement_1), pp.S83-S89
01/01/1989
DOI: 10.1093/clinids/11.Supplement_1.S83
PMID: 2564693
Abstract
The primary cause of toxic shock syndrome is toxic shock syndrome toxin 1 (TSST-1), a 22,049-dalton exotoxin. Approximately 20% of Staphylococcus aureus isolates produce TSST-1; the production of this toxin is therefore a variable genetic trait. The TSST-1 gene and its flanking sequences are found on a genetic element that is present in TSST-1-positive isolates and absent in TSST-1-negative isolates. Preliminary sequence data and Southern hybridization experiments with the cloned flanking sequences have provided evidence that the TSST-1 element is 4-7 kilobases in size. Hybridization analysis of whole-cell DNA from two genetically mapped TSST-1-positive strains has demonstrated that the TSST-1 element has at least two chromosomal locations. This finding suggests that the element is mobile. Biotyping of 75 TSST-1-positive isolates showed that the large majority were tryptophan-negative, and Southern hybridization analysis of whole-cell DNA from these isolates revealed a common blotting pattern--an observation suggesting that these strains are clonal.
Details
- Title: Subtitle
- Toxic Shock Syndrome Toxin 1 Is Encoded by a Variable Genetic Element
- Creators
- Barry N KreiswirthSteven J ProjanPatrick M SchlievertRichard P Novick
- Resource Type
- Journal article
- Publication Details
- Clinical infectious diseases, Vol.11(Supplement_1), pp.S83-S89
- DOI
- 10.1093/clinids/11.Supplement_1.S83
- PMID
- 2564693
- ISSN
- 1058-4838
- eISSN
- 1537-6591
- Language
- English
- Date published
- 01/01/1989
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001108402771
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