Toxicity Impacts on Human Adipose Mesenchymal Stem/Stromal Cells Acutely Exposed to Aroclor and Non-Aroclor Mixtures of Polychlorinated Biphenyl

Riley M Behan-Bush; Jesse N Liszewski; Michael V Schrodt; Bhavya Vats; Xueshu Li; Hans-Joachim Lehmler; Aloysius J Klingelhutz; James A Ankrum

doi:10.1021/acs.est.2c07281

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Toxicity Impacts on Human Adipose Mesenchymal Stem/Stromal Cells Acutely Exposed to Aroclor and Non-Aroclor Mixtures of Polychlorinated Biphenyl

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Toxicity Impacts on Human Adipose Mesenchymal Stem/Stromal Cells Acutely Exposed to Aroclor and Non-Aroclor Mixtures of Polychlorinated Biphenyl

Riley M Behan-Bush, Jesse N Liszewski, Michael V Schrodt, Bhavya Vats, Xueshu Li, Hans-Joachim Lehmler, Aloysius J Klingelhutz and James A Ankrum

Environmental science & technology, Vol.57(4), pp.1731-1742

01/31/2023

DOI: 10.1021/acs.est.2c07281

PMCID: PMC9893815

PMID: 36651682

Appears in UI Libraries Support Open Access

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Abstract

Polychlorinated biphenyl (PCB) accumulates in adipose where it may impact the growth and function of cells within the tissue. This is particularly concerning during adolescence when adipocytes expand rapidly. Herein, we sought to understand how exposure to PCB mixtures found in U.S. schools affects human adipose mesenchymal stem/stromal cell (MSC) health and function. We investigated how exposure to Aroclor 1016 and Aroclor 1254, as well as a newly characterized non-Aroclor mixture that resembles the PCB profile found in cabinets, Cabinet Mixture, affects adipose MSC growth, viability, and function in vitro. We found that exposure to all three mixtures resulted in two distinct types of toxicity. At PCB concentrations >20 μM, the majority of MSCs die, while at 1-10 μM, MSCs remained viable but display numerous alterations to their phenotype. At these sublethal concentrations, the MSC rate of expansion slowed and morphology changed. Further assessment revealed that PCB-exposed MSCs had impaired adipogenesis and a modest decrease in immunosuppressive capabilities. Thus, exposure to PCB mixtures found in schools negatively impacts the health and function of adipose MSCs. This work has implications for human health due to MSCs' role in supporting the growth and maintenance of adipose tissue.

mesenchymal stem cell

persistent organic pollutants

endocrine disrupting chemical

EDC

UIOWA OA Agreement

Details

Title: Subtitle: Toxicity Impacts on Human Adipose Mesenchymal Stem/Stromal Cells Acutely Exposed to Aroclor and Non-Aroclor Mixtures of Polychlorinated Biphenyl
Creators: Riley M Behan-Bush - University of Iowa
Jesse N Liszewski - University of Iowa
Michael V Schrodt - University of Iowa
Bhavya Vats - University of Iowa
Xueshu Li - University of Iowa
Hans-Joachim Lehmler - University of Iowa
Aloysius J Klingelhutz - University of Iowa
James A Ankrum - University of Iowa
Resource Type: Journal article
Publication Details: Environmental science & technology, Vol.57(4), pp.1731-1742
DOI: 10.1021/acs.est.2c07281
PMID: 36651682
PMCID: PMC9893815
NLM abbreviation: Environ Sci Technol
ISSN: 0013-936X
eISSN: 1520-5851
Publisher: American Chemical Society
Grant note: DOI: 10.13039/100000066, name: U.S. Department of Health and Human Services, award: P30 ES005605, P42 ES013661; DOI: 10.13039/100000054, name: U.S. Department of Health and Human Services, award: P30CA086862
Language: English
Electronic publication date: 01/18/2023
Date published: 01/31/2023
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Microbiology and Immunology; Occupational and Environmental Health; Iowa Technology Institute; Iowa Neuroscience Institute; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Iowa Superfund Research Program
Record Identifier: 9984360858202771

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