Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies

Sudartip Areecheewakul; Andrea Adamcakova-Dodd; Brittany E Givens; Benjamin R Steines; Yifang Wang; David K Meyerholz; Nathanial J Parizek; Ralph Altmaier; Ezazul Haque; Patrick T O'Shaughnessy; Aliasger K Salem; Peter S Thorne

doi:10.1016/j.impact.2020.100214

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Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies

Journal article

Open access

Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies

Sudartip Areecheewakul, Andrea Adamcakova-Dodd, Brittany E Givens, Benjamin R Steines, Yifang Wang, David K Meyerholz, Nathanial J Parizek, Ralph Altmaier, Ezazul Haque, Patrick T O'Shaughnessy, …

NanoImpact, Vol.18, p.100214

04/2020

DOI: 10.1016/j.impact.2020.100214

PMID: 32968700

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https://www.ncbi.nlm.nih.gov/pmc/articles/7504913View

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Abstract

Characterizations and in vitro toxicity screening were performed on metal oxide engineered nanomaterials (ENMs) independently comprising ZnO, CuO, CeO2, Fe2O3, WO3, V2O5, TiO2, Al2O3 and MgO. Nanomaterials that exhibited the highest toxicity responses in the in vitro screening assays (ZnO, CuO, and V2O5) and the lesser explored material WO3 were tested for acute pulmonary toxicity in vivo. Female and male mice (C57Bl/6J) were exposed to aerosolized metal oxide ENMs in a nose-only exposure system and toxicity outcomes (biomarkers of cytotoxicity, immunotoxicity, inflammation, and lung histopathology) at 4 and 24 h after the start of exposure were assessed. The studies were performed as part of the NIEHS Nanomaterials Health Implications Research consortium with the purpose of investigating the effects of ENMs on various biological systems. ENMs were supplied by the Engineered Nanomaterials Resource and Coordination Core. Among the ENMs studied, the highest toxicity was observed for CuO and ZnO NPs in both in vitro and in vivo acute models. Compared to sham-exposed controls, there was a significant increase in bronchoalveolar lavage neutrophils and proinflammatory cytokines and a loss of macrophage viability at both 4 h and 24 h for ZnO and CuO but not seen for V2O5 or WO3. These effects were observed in both female and male mice. The cell viability performed after in vitro exposure to ENMs and assessment of lung inflammation after acute inhalation exposure in vivo were shown to be sensitive endpoints to predict ENM acute toxicity. [Display omitted] •Acute in vitro and in vivo toxicity screening methods were evaluated using metal oxide engineered nanomaterials (ENMs).•ZnO, CuO and V2O5 exhibited significant in vitro toxicity responses while CeO2, Fe2O3, WO3, TiO2, Al2O3 and MgO were low.•The highest toxicity was seen for CuO and ZnO ENMs in acute nose-only exposure studies of male and female C57Bl/6J mice.

Nanoparticles

Inhalation toxicity

Metal oxides

Nanomaterials

In vitro toxicity

Details

Title: Subtitle: Toxicity assessment of metal oxide nanomaterials using in vitro screening and murine acute inhalation studies
Creators: Sudartip Areecheewakul - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa, Iowa City, IA 52246, USA
Andrea Adamcakova-Dodd - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USA
Brittany E Givens - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa, Iowa City, IA 52246, USA
Benjamin R Steines - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USA
Yifang Wang - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, Iowa City, IA 52246, USA
David K Meyerholz - Department of Pathology, University of Iowa, Iowa City, IA 52246, USA
Nathanial J Parizek - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, Iowa City, IA 52246, USA
Ralph Altmaier - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USA
Ezazul Haque - Interdisciplinary Graduate Program in Human Toxicology, University of Iowa, Iowa City, IA 52246, USA
Patrick T O'Shaughnessy - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USA
Aliasger K Salem - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa, Iowa City, IA 52246, USA
Peter S Thorne - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52246, USA
Resource Type: Journal article
Publication Details: NanoImpact, Vol.18, p.100214
DOI: 10.1016/j.impact.2020.100214
PMID: 32968700
NLM abbreviation: NanoImpact
ISSN: 2452-0748
eISSN: 2452-0748
Publisher: Elsevier B.V
Grant note: DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: U01ES027252; DOI: 10.13039/100000002, name: National Institutes of Health; DOI: 10.13039/100000879, name: Alfred P. Sloan Foundation; name: National GEM Consortium Associate Fellowship; DOI: 10.13039/100008893, name: University of Iowa; name: Lyle & Sharon Bighley Endowed Professorship
Language: English
Date published: 04/2020
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics; Pathology; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9984001078402771

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