Journal article
Trace quantitation of 4-hydroxy-2-nonenal in biological samples as its oxime—bis- tert.-butyldimethylsilyl derivative using 3-hydroxynonanal as an internal standard
Journal of chromatography. Biomedical applications, Vol.578(1), pp.9-16
1992
DOI: 10.1016/0378-4347(92)80219-G
PMID: 1400791
Abstract
A gas chromatographic—mass spectrometric method for the determination of the lipid aldehyde 4-hydroxy-2-nonenal (4HNE) in trace quantities is described. The method utilizes the reaction of aldehydes with hydroxylamine leading to the formation of the oxime derivative. The aldehydes are recovered by octadecylsilyl solid-phase extraction and converted to the bis-
tert.-butyldimethylsilyl derivatives for analysis using electron ionization. A novel 4HNE analogue, 3-hydroxynonanal, has been synthesized and is used as an internal standard. A limit of detection of approximately 1 pmol of 4 HNE in preparations of approximately 2 · 10
6 cells or 0.5 ml of whole blood, plasma or serum was observed. Standard addition analysis indicates that the method is accurate at these levels. Replicate analysis of the National Institutes of Standards and Technology Standard Reference Material SRM 909 indicates an average in-run precision of 8.1% and a between-run precision of 13.5% at an average concentration of 82.1 pmol/ml of reconstituted material.
Details
- Title: Subtitle
- Trace quantitation of 4-hydroxy-2-nonenal in biological samples as its oxime—bis- tert.-butyldimethylsilyl derivative using 3-hydroxynonanal as an internal standard
- Creators
- Michael Kinter - University of VirginiaShannon Sullivan - University of VirginiaRobert J. Roberts - University of VirginiaDouglas Spitz - University of Virginia
- Resource Type
- Journal article
- Publication Details
- Journal of chromatography. Biomedical applications, Vol.578(1), pp.9-16
- DOI
- 10.1016/0378-4347(92)80219-G
- PMID
- 1400791
- NLM abbreviation
- J Chromatogr B Biomed Appl
- ISSN
- 0378-4347
- eISSN
- 1872-812X
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1992
- Academic Unit
- Stead Family Department of Pediatrics; Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984313068602771
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