Journal article
Tracking embryonic hematopoietic stem cells to the bone marrow: nanoparticle options to evaluate transplantation efficiency
Stem Cell Research & Therapy, Vol.9(1), 204
07/27/2018
DOI: 10.1186/s13287-018-0944-8
PMCID: PMC6062968
PMID: 30053892
Abstract
Background:
As the prevalence of therapeutic approaches involving transplanted cells increases, so does the need to noninvasively track the cells to determine their homing patterns. Of particular interest is the fate of transplanted embryonic stem cell-derived hematopoietic progenitor cells (HPCs) used to restore the bone marrow pool following sublethal myeloablative irradiation. The early homing patterns of cell engraftment are not well understood at this time. Until now, longitudinal studies were hindered by the necessity to sacrifice several mice at various time points of study, with samples of the population of lymphoid compartments subsequently analyzed by flow cytometry or fluorescence microscopy. Thus, long-term study and serial analysis of the transplanted cells within the same animal was cumbersome, making difficult an accurate documentation of engraftment, functionality, and cell reconstitution patterns.
Methods:
Here, we devised a noninvasive, nontoxic modality for tracking early HPC homing patterns in the same mice longitudinally over a period of 9 days using mesoporous silica nanoparticles (MSNs) and magnetic resonance imaging.
Results:
This approach of potential translational importance helps to demonstrate efficient uptake of MSNs by the HPCs as well as retention of MSN labeling in vivo as the cells were traced through various organs, such as the spleen, bone marrow, and kidney. Altogether, early detection of the whereabouts and engraftment of transplanted stem cells may be important to the overall outcome. To accomplish this, there is a need for the development of new noninvasive tools.
Conclusions:
Our data suggest that multifunctional MSNs can label viably blood-borne HPCs and may help document the distribution and homing in the host followed by successful reconstitution.
Details
- Title: Subtitle
- Tracking embryonic hematopoietic stem cells to the bone marrow: nanoparticle options to evaluate transplantation efficiency
- Creators
- Sean K Sweeney - University of Iowa, Roy J. Carver Department of Biomedical EngineeringGohar S. Manzar - Department of Biomedical Engineering, University of IowaNicholas Zavazava - University of Iowa, Roy J. Carver Department of Biomedical EngineeringJose G. Assouline - University of Iowa, Roy J. Carver Department of Biomedical Engineering
- Resource Type
- Journal article
- Publication Details
- Stem Cell Research & Therapy, Vol.9(1), 204
- Publisher
- BMC
- DOI
- 10.1186/s13287-018-0944-8
- PMID
- 30053892
- PMCID
- PMC6062968
- ISSN
- 1757-6512
- eISSN
- 1757-6512
- Copyright
- © 2018, Springer Nature
- Grant note
- Funding: The work is funded by Iowa Economic Development Authority Demonstration Fund #13-DEMO-005, NSF SBIR grant #IIP-1345646, and VA grant 5I01BX001125–03. Competing Interests: JGA is founder and sole proprietor of and SKS is an employee of NanoMedTrix, LLC, which prepared the nanoparticles used in this study. All results and claims have been independently verified by other scientists with no financial stake in the company. The authors declare that they have no competing interests.
- Language
- English
- Date published
- 07/27/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9983761198102771
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