Journal article
Tracking mesenchymal stem cells with iron oxide nanoparticle loaded poly(lactide-co-glycolide) microparticles
Nano letters, Vol.12(8), pp.4131-4139
08/08/2012
DOI: 10.1021/nl301658q
PMCID: PMC3552518
PMID: 22769232
Abstract
Monitoring the location, distribution and long-term engraftment of administered cells is critical for demonstrating the success of a cell therapy. Among available imaging-based cell tracking tools, magnetic resonance imaging (MRI) is advantageous due to its noninvasiveness, deep penetration, and high spatial resolution. While tracking cells in preclinical models via internalized MRI contrast agents (iron oxide nanoparticles, IO-NPs) is a widely used method, IO-NPs suffer from low iron content per particle, low uptake in nonphagocytotic cell types (e.g., mesenchymal stem cells, MSCs), weak negative contrast, and decreased MRI signal due to cell proliferation and cellular exocytosis. Herein, we demonstrate that internalization of IO-NP (10 nm) loaded biodegradable poly(lactide-co-glycolide) microparticles (IO/PLGA-MPs, 0.4-3 μm) in MSCs enhances MR parameters such as the r(2) relaxivity (5-fold), residence time inside the cells (3-fold) and R(2) signal (2-fold) compared to IO-NPs alone. Intriguingly, in vitro and in vivo experiments demonstrate that internalization of IO/PLGA-MPs in MSCs does not compromise inherent cell properties such as viability, proliferation, migration and their ability to home to sites of inflammation.
Details
- Title: Subtitle
- Tracking mesenchymal stem cells with iron oxide nanoparticle loaded poly(lactide-co-glycolide) microparticles
- Creators
- Chenjie Xu - Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Harvard Stem Cell Institute, Harvard-MIT, Division of Health Sciences and Technology, 65 Landsdowne Street, Cambridge, Massachusetts 02139, USADavid Miranda-NievesJames A AnkrumMads Emil MatthiesenJoseph A PhillipsIsaac RoesGregory R WojtkiewiczVikram JunejaJens Roat KultimaWeian ZhaoPraveen Kumar VemulaCharles P LinMatthias NahrendorfJeffrey M Karp
- Resource Type
- Journal article
- Publication Details
- Nano letters, Vol.12(8), pp.4131-4139
- DOI
- 10.1021/nl301658q
- PMID
- 22769232
- PMCID
- PMC3552518
- NLM abbreviation
- Nano Lett
- ISSN
- 1530-6984
- eISSN
- 1530-6992
- Publisher
- United States
- Grant note
- R01 HL095722 / NHLBI NIH HHS HL095722 / NHLBI NIH HHS HL097172 / NHLBI NIH HHS HHSN268201000044C / NHLBI NIH HHS R21 HL097172 / NHLBI NIH HHS HHSN268201000044C / PHS HHS
- Language
- English
- Date published
- 08/08/2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984000919102771
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