Journal article
Traffic into the prevacuolar/endosomal compartment of Saccharomyces cerevisiae: A VPS45-dependent intracellular route and a VPS45-independent, endocytic route
European journal of cell biology, Vol.76(1), pp.43-52
1998
DOI: 10.1016/S0171-9335(98)80016-2
PMID: 9650782
Abstract
The
vps (
vacuolar
protein
sorting) mutants have been used to dissect and charaterize the vacuolar biogenesis pathway in the yeast
Saccharomyces cerevisiae. The
vps mutants were isolated through their loss of ability to correctly sort the vacuolar hydrolase CPY, which travels from Golgi membranes to the vacuole through a prevacuolar compartment. Over 50
VPS genes have been divided into 6 classes according to vacuolar morphology. Mutations in any one of the class E
VPS genes, such as
VPS27, lead to an exaggerated form of the prevacuolar compartment. This class E compartment contains endocytosed proteins as well as proteins en route to the vacuole, and is thus taken to represent an intersection point between the endocytic and biosynthetic pathways. Mutations in the class D gene
VPS45 can be used to define a second transport intermediate along the vacuolar biogenesis pathway, Golgi-derived transport vesicles carrying vacuolar membrane proteins on their way to the vacuole. Here we demonstrate that the Sec1p-like protein Vps45p is required for the fusion of Golgi-derived vesicles with the prevacuolar compartment indicating that
VPS45 functions before
VPS27 in the vacuolar biogenesis pathway. In addition, we show that
VPS45 function is not required for the delivery of endocytosed proteins to the prevacuolar compartment from the plasma membrane suggesting that the function of Vps45p is restricted to a single vesicular pathway.
Details
- Title: Subtitle
- Traffic into the prevacuolar/endosomal compartment of Saccharomyces cerevisiae: A VPS45-dependent intracellular route and a VPS45-independent, endocytic route
- Creators
- Nia J. Bryant - University of OregonRobert C. Piper - University of IowaSonja R. Gerrard - University of OregonTom H. Stevens - University of Oregon
- Resource Type
- Journal article
- Publication Details
- European journal of cell biology, Vol.76(1), pp.43-52
- Publisher
- Elsevier GmbH
- DOI
- 10.1016/S0171-9335(98)80016-2
- PMID
- 9650782
- ISSN
- 0171-9335
- eISSN
- 1618-1298
- Language
- English
- Date published
- 1998
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297511802771
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