Journal article
Transcription Factor AP-2 Is an Essential and Direct Regulator of Epidermal Development in Xenopus
Developmental biology, Vol.245(1), pp.136-144
05/01/2002
DOI: 10.1006/dbio.2002.0621
PMID: 11969261
Abstract
Expression of the Xenopus homolog of the mammalian transcription factor AP-2α (XAP-2) is activated throughout the animal hemisphere shortly after the midblastula transition, and becomes restricted to prospective epidermis by the end of gastrulation, under the control of BMP signal modulation. Elevated expression in the future neural crest region begins at this time. Ectopic expression of XAP-2 can restore transcription of epidermal genes in neuralized ectoderm, both in ectodermal explants and in the intact embryo. Likewise, loss of XAP-2 function, accomplished by injection of antisense oligonucleotides or by overexpression of antimorphic XAP-2 derivatives, leads to loss of epidermal and gain of neural gene expression. These treatments also result in gastrulation failure. Thus, AP-2 is a critical regulator of ectodermal determination that is required for normal epidermal development and morphogenesis in the frog embryo.
Details
- Title: Subtitle
- Transcription Factor AP-2 Is an Essential and Direct Regulator of Epidermal Development in Xenopus
- Creators
- Ting Luo - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20892Mami Matsuo-Takasaki - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20892Megan L Thomas - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20892Daniel L Weeks - Department of Biochemistry, University of Iowa, Iowa City, Iowa, 52242Thomas D Sargent - Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20892
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.245(1), pp.136-144
- DOI
- 10.1006/dbio.2002.0621
- PMID
- 11969261
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 05/01/2002
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology
- Record Identifier
- 9984024505502771
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