Journal article
Transcription Profiling of Epstein-Barr Virus Nuclear Antigen (EBNA)-1 Expressing Cells Suggests Targeting of Chromatin Remodeling Complexes
PloS one, Vol.5(8), pp.e12052-e12052
08/10/2010
DOI: 10.1371/journal.pone.0012052
PMCID: PMC2919392
PMID: 20706582
Abstract
The Epstein-Barr virus (EBV) encoded nuclear antigen (EBNA)-1 regulates virus replication and transcription, and participates in the remodeling of the cellular environment that accompanies EBV induced B-cell immortalization and malignant transformation. The putative cellular targets of these effects of EBNA-1 are largely unknown. To address this issue we have profiled the transcriptional changes induced by short-and long-term expression of EBNA-1 in the EBV negative B-cell lymphoma BJAB. Three hundred and nineteen cellular genes were regulated in a conditional transfectant shortly after EBNA-1 induction while a ten fold higher number of genes was regulated upon continuous EBNA-1 expression. Promoter analysis of the differentially regulated genes demonstrated a significant enrichment of putative EBNA-1 binding sites suggesting that EBNA-1 may directly influence the transcription of a subset of genes. Gene ontology analysis of forty seven genes that were consistently regulated independently on the time of EBNA-1 expression revealed an unexpected enrichment of genes involved in the maintenance of chromatin architecture. The interaction network of the affected gene products suggests that EBNA-1 may promote a broad rearrangement of the cellular transcription landscape by altering the expression of key components of chromatin remodeling complexes.
Details
- Title: Subtitle
- Transcription Profiling of Epstein-Barr Virus Nuclear Antigen (EBNA)-1 Expressing Cells Suggests Targeting of Chromatin Remodeling Complexes
- Creators
- Ramakrishna Sompallae - Karolinska InstitutetSimone Callegari - Karolinska InstitutetSiamak Akbari Kamranvar - Karolinska InstitutetMaria G. Masucci - Karolinska Institutet
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.5(8), pp.e12052-e12052
- Publisher
- Public Library Science
- DOI
- 10.1371/journal.pone.0012052
- PMID
- 20706582
- PMCID
- PMC2919392
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Number of pages
- 10
- Grant note
- Swedish Cancer Society LSHC-CT-2005-018704 / European Community; European Commission Swedish Medical Research Council; Swedish Medical Research Council (SMRC) Karolinska Institutet, Stockholm, Sweden
- Language
- English
- Date published
- 08/10/2010
- Academic Unit
- Pathology
- Record Identifier
- 9984622752602771
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