Journal article
Transcription elongation factor P-TEFb is required for HIV-1 Tat transactivation in vitro
Genes & development, Vol.11(20), pp.2622-2632
10/15/1997
DOI: 10.1101/gad.11.20.2622
PMCID: PMC316609
PMID: 9334325
Abstract
P-TEFb is a key regulator of the process controlling the processivity of RNA polymerase II and possesses a kinase activity that can phosphorylate the carboxy-terminal domain of the largest subunit of RNA polymerase II. Here we report the cloning of the small subunit of
Drosophila
P-TEFb and the finding that it encodes a Cdc2-related protein kinase. Sequence comparison suggests that a protein with 72% identity, PITALRE, could be the human homolog of the
Drosophila
protein. Functional homology was suggested by transcriptional analysis of an RNA polymerase II promoter with HeLa nuclear extract depleted of PITALRE. Because the depleted extract lost the ability to produce long DRB-sensitive transcripts and this loss was reversed by the addition of purified
Drosophila
P-TEFb, we propose that PITALRE is a component of human P-TEFb. In addition, we found that PITALRE associated with the activation domain of HIV-1 Tat, indicating that P-TEFb is a Tat-associated kinase (TAK). An in vitro transcription assay demonstrates that the effect of Tat on transcription elongation requires P-TEFb and suggests that the enhancement of transcriptional processivity by Tat is attributable to enhanced function of P-TEFb on the HIV-1 LTR.
Details
- Title: Subtitle
- Transcription elongation factor P-TEFb is required for HIV-1 Tat transactivation in vitro
- Creators
- Yuerong Zhu - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USATsafrira Pe’ery - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USAJunmin Peng - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USAYegnanarayana Ramanathan - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USANick Marshall - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USATricia Marshall - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USABrad Amendt - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USAMichael B Mathews - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USADavid H Price - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242 USA
- Resource Type
- Journal article
- Publication Details
- Genes & development, Vol.11(20), pp.2622-2632
- DOI
- 10.1101/gad.11.20.2622
- PMID
- 9334325
- PMCID
- PMC316609
- NLM abbreviation
- Genes Dev
- ISSN
- 0890-9369
- eISSN
- 1549-5477
- Publisher
- Cold Spring Harbor Laboratory Press
- Language
- English
- Date published
- 10/15/1997
- Academic Unit
- Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Biochemistry and Molecular Biology; Dental Research
- Record Identifier
- 9984024417202771
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