Transcriptional Regulation by Lge1p Requires a Function Independent of Its Role in Histone H2B Ubiquitination

Xiaoting Zhang; Ania Kolaczkowska; Frédéric Devaux; Sneh Lata Panwar; Timothy C. Hallstrom; Claude Jacq; W. Scott Moye-Rowley

doi:10.1074/jbc.M408333200

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Transcriptional Regulation by Lge1p Requires a Function Independent of Its Role in Histone H2B Ubiquitination

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Transcriptional Regulation by Lge1p Requires a Function Independent of Its Role in Histone H2B Ubiquitination

Xiaoting Zhang, Ania Kolaczkowska, Frédéric Devaux, Sneh Lata Panwar, Timothy C. Hallstrom, Claude Jacq and W. Scott Moye-Rowley

The Journal of biological chemistry, Vol.280(4), pp.2759-2770

01/28/2005

DOI: 10.1074/jbc.M408333200

PMID: 15528187

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Abstract

Saccharomyces cerevisiae cells that have lost their mitochondrial genome (ρ0) strongly induce transcription of multidrug resistance genes, including the ATP-binding cassette transporter gene PDR5. PDR5 induction in ρ0 cells requires the presence of the zinc cluster transcription factor Pdr3p. The PDR3 gene is positively autoregulated in ρ0 cells by virtue of the presence of two binding sites for Pdr3p in its promoter. We identify the novel protein Lge1p as a required participant in the ρ0 activation of PDR3 and PDR5 expression. Lge1p is a nuclear protein that has been found to play a role in ubiquitination of histone H2B at Lys123. This ubiquitination requires the presence of the ubiquitin-conjugating enzyme Rad6p and the ubiquitin ligase Bre1p. Interestingly, ρ0 strains lacking Lge1p failed to induce PDR3 transcription, but induction was still seen in Δrad6, Δbre1, and H2B-K123R mutant strains. Microarray experiments also confirmed that the pattern of gene expression changes seen in cells lacking Lge1p, Bre1p, or Rad6p or containing the H2B-K123R mutant as the only form of H2B share some overlap but are distinct. These findings provide a strong argument that Lge1p has roles in gene regulation independent of its participation in the Rad6p-dependent ubiquitination of H2B.

Details

Title: Subtitle: Transcriptional Regulation by Lge1p Requires a Function Independent of Its Role in Histone H2B Ubiquitination
Creators: Xiaoting Zhang - University of Iowa
Ania Kolaczkowska - University of Iowa
Frédéric Devaux - Laboratoire de Génétique Moléculaire, CNRS, Ecole Normale Supérieure, 75230 Paris, Cedex 05, France
Sneh Lata Panwar - University of Iowa
Timothy C. Hallstrom - University of Iowa
Claude Jacq - Laboratoire de Génétique Moléculaire, CNRS, Ecole Normale Supérieure, 75230 Paris, Cedex 05, France
W. Scott Moye-Rowley - University of Iowa
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.280(4), pp.2759-2770
DOI: 10.1074/jbc.M408333200
PMID: 15528187
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: Elsevier Inc
Language: English
Date published: 01/28/2005
Academic Unit: Molecular Physiology and Biophysics; Internal Medicine
Record Identifier: 9984297511002771

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