Journal article
Transcriptional Regulation of CLN3 Expression by Glucose in Saccharomyces cerevisiae
Journal of bacteriology, Vol.180(17), pp.4508-4515
09/1998
DOI: 10.1128/jb.180.17.4508-4515.1998
PMCID: PMC107461
PMID: 9721289
Abstract
In
Saccharomyces cerevisiae
, the transition from the G
1
phase of the mitotic cycle into S phase is controlled by a set of G
1
cyclins that regulate the activity of the protein kinase encoded by
CDC28
. Yeast cells regulate progress through the G
1
/S boundary in response to nutrients, moving quickly through G
1
in glucose medium and more slowly in poorer medium. We have examined connections between glucose and the level of the message encoding Cln3, a G
1
cyclin. We found that glucose positively regulates
CLN3
mRNA levels through a set of repeated AAGAAAAA (A
2
GA
5
) elements within the
CLN3
promoter. Mutations in these sequences reduce both transcriptional activation and specific interaction between
CLN3
promoter elements and proteins in yeast extracts. Creation of five point mutations, replacing the G’s within these repeats with T’s, in the
CLN3
promoter substantially reduces
CLN3
expression in glucose medium and inhibits the ability of the cells to maintain a constant size when shifted into glucose.
Details
- Title: Subtitle
- Transcriptional Regulation of CLN3 Expression by Glucose in Saccharomyces cerevisiae
- Creators
- Fereshteh Parviz - School of PharmacyDuane D Hall - School of PharmacyDavid D Markwardt - School of PharmacyWarren Heideman - School of Pharmacy
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.180(17), pp.4508-4515
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/jb.180.17.4508-4515.1998
- PMID
- 9721289
- PMCID
- PMC107461
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Language
- English
- Date published
- 09/1998
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984094587602771
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