Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4

Keith D. Harshman; W.Scott Moye-Rowley; Carl S. Parker

doi:10.1016/0092-8674(88)90393-5

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Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4

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Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4

Keith D. Harshman, W.Scott Moye-Rowley and Carl S. Parker

Cell, Vol.53(2), pp.321-330

04/22/1988

DOI: 10.1016/0092-8674(88)90393-5

PMID: 2834068

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Abstract

The consensus recognition element for the mammalian transcription factor AP-1 is very similar to that of the transcriptional activator GCN4. Here, we show that the AP-1 recognition element (ARE) found in the SV40 enhancer can activate transcription from a heterologous promoter in S. cerevisiae. This activation, however, is not dependent on the presence of GCN4 as evidenced by ARE-dependent transcription in a gcn4 yeast strain. A previously unknown yeast transcription factor that is probably responsible for this activation was identified and highly purified. The yeast factor, designated yAP-1, shares remarkably similar biochemical and DNA-binding characteristics with mammalian AP-1. These data suggest that the yeast and mammalian AP-1 are evolutionarily conserved and perhaps functionally related. Also note-worthy is that GCN4 can bind to a GCN4 recognition element (GCRE) and to the ARE with approximately equal affinities; yAP-1, however, has a much lower affinity for the GCRE than the ARE, suggesting that yAP-1 can discriminate between these elements in vivo.

Details

Title: Subtitle: Transcriptional activation by the SV40 AP-1 recognition element in yeast is mediated by a factor similar to AP-1 that is distinct from GCN4
Creators: Keith D. Harshman - California Institute of Technology
W.Scott Moye-Rowley - California Institute of Technology
Carl S. Parker - California Institute of Technology
Resource Type: Journal article
Publication Details: Cell, Vol.53(2), pp.321-330
Publisher: Elsevier Inc
DOI: 10.1016/0092-8674(88)90393-5
PMID: 2834068
ISSN: 0092-8674
eISSN: 1097-4172
Language: English
Date published: 04/22/1988
Academic Unit: Molecular Physiology and Biophysics; Internal Medicine
Record Identifier: 9984297602002771

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