Journal article
Transcriptional control of estrogen receptor in estrogen receptor-negative breast carcinoma
Cancer research (Chicago, Ill.), Vol.53(15), pp.3472-3474
1993
PMID: 8339250
Abstract
The mechanisms which control estrogen receptor (ER) expression in breast carcinoma have not been elucidated. The MCF-7 (ER-positive) and MDA-MB-231 (ER-negative) breast carcinoma cell lines have been used to examine ER gene expression. Northern blot analysis of mRNA isolated from these two cell lines demonstrates that MCF-7 cells express an expected 6.5-kilobase ER mRNA whereas MDA-MB-231 do not make detectable ER message. Reverse polymerase chain reaction, which offers greater sensitivity, confirms these Northern blot results. The nuclear run-on assay was used to examine directly transcription in these two cell lines. MCF-7 cells actively transcribe the ER gene whereas no ER transcription is detected in MDA-MB-231. Southern analysis of genomic DNA confirms that the ER gene is present in MDA-MB-231. We conclude that the ER gene is controlled transcriptionally in this ER-negative breast carcinoma line.
Details
- Title: Subtitle
- Transcriptional control of estrogen receptor in estrogen receptor-negative breast carcinoma
- Creators
- R. J Weigel - Stanford UniversityE. C Deconinck - Stanford University
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.53(15), pp.3472-3474
- Publisher
- American Association for Cancer Research
- PMID
- 8339250
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 1993
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology; Surgery; Biochemistry and Molecular Biology
- Record Identifier
- 9984284338802771
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