Transcriptional profile of Rous Sarcoma Virus transformed chicken embryo fibroblasts reveals new signaling targets of viral- src

Kathryn Masker; Alicia Golden; Christian J Gaffney; Virginia Mazack; William F Schwindinger; Weizhou Zhang; Lu-Hai Wang; David J Carey; Marius Sudol

doi:10.1016/j.virol.2007.03.026

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Transcriptional profile of Rous Sarcoma Virus transformed chicken embryo fibroblasts reveals new signaling targets of viral- src

Kathryn Masker, Alicia Golden, Christian J Gaffney, Virginia Mazack, William F Schwindinger, Weizhou Zhang, Lu-Hai Wang, David J Carey and Marius Sudol

Virology (New York, N.Y.), Vol.364(1), pp.10-20

2007

DOI: 10.1016/j.virol.2007.03.026

PMCID: PMC1974879

PMID: 17448514

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Abstract

Transformation of chicken fibroblasts in vitro by Rous Sarcoma Virus represents a model of cancer in which a single oncogene, viral src, uniformly and rapidly transforms primary cells in culture. We experimentally surveyed the transcriptional program affected by Rous Sarcoma Virus (RSV) in primary culture of chicken embryo fibroblasts. As a control, we used cells infected with non-transforming RSV mutant td106, in which the src gene was deleted. Using Affymetrix GeneChip® Chicken Genome Arrays, we report 811 genes that were modulated more than 2.5 fold in the virus transformed cells. Among these, 409 genes were induced and 402 genes were repressed by viral src. From the repertoire of modulated genes, we selected 20 genes that were robustly changed. We then validated and quantified the transcriptional changes of most of the 20 selected genes by real-time PCR. The set of strongly induced genes contains vasoactive intestinal polypeptide, MAP kinase phosphatase 2 and follistatin, among others. The set of strongly repressed genes contains TGF beta 3, TGF beta-induced gene, and deiodinase. The function of several robustly modulated genes sheds new light on the molecular mechanism of oncogenic transformation.

Follistatin

Schmidt-Ruppin strain of RSV

Vasoactive intestinal polypeptide

Avian sarcoma

src oncogene

Protein-tyrosine kinase

MAP kinase phosphatase 2

Details

Title: Subtitle: Transcriptional profile of Rous Sarcoma Virus transformed chicken embryo fibroblasts reveals new signaling targets of viral- src
Creators: Kathryn Masker - Weis Center for Research, Danville, PA, USA
Alicia Golden - Weis Center for Research, Danville, PA, USA
Christian J Gaffney - Weis Center for Research, Danville, PA, USA
Virginia Mazack - Weis Center for Research, Danville, PA, USA
William F Schwindinger - Weis Center for Research, Danville, PA, USA
Weizhou Zhang - Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
Lu-Hai Wang - Department of Microbiology, Mount Sinai School of Medicine, New York, NY, USA
David J Carey - Weis Center for Research, Danville, PA, USA
Marius Sudol - Weis Center for Research, Danville, PA, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.364(1), pp.10-20
DOI: 10.1016/j.virol.2007.03.026
PMID: 17448514
PMCID: PMC1974879
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Language: English
Date published: 2007
Academic Unit: Radiation Oncology
Record Identifier: 9984083886902771

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