Transcriptional profiling of lymphoblast lines from subjects with panic disorder

Robert A Philibert; Raymond Crowe; Gi-Yung Ryu; Jae-Geun Yoon; Dianna Secrest; Harinder Sandhu; Anup Madan

doi:10.1002/ajmg.b.30502

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Transcriptional profiling of lymphoblast lines from subjects with panic disorder

Journal article

Peer reviewed

Transcriptional profiling of lymphoblast lines from subjects with panic disorder

Robert A Philibert, Raymond Crowe, Gi-Yung Ryu, Jae-Geun Yoon, Dianna Secrest, Harinder Sandhu and Anup Madan

American journal of medical genetics. Part B, Neuropsychiatric genetics, Vol.144B(5), pp.674-682

07/05/2007

DOI: 10.1002/ajmg.b.30502

PMID: 17342723

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Abstract

In attempts to isolate genetic vulnerability factors for panic disorder (PD), a number of investigators have used genome-wide linkage or association analyses. But these attempts have been only modestly successful which suggests that alternative approaches may be needed to define the biology of PD. Therefore, using recently developed genome-wide gene expression profiling, we explored whether transcriptional signatures associated with PD are present in lymphoblast cell line. The expression of 2,469 transcripts in lymphoblast cell lines from 16 subjects was arithmetically increased in every line and significantly increased overall and 354 transcripts was arithmetically decreased in every cell line and significantly decreased overall as compared to those lymphoblast lines from 17 subjects without a history of behavioral illness. Further sex specific analyses showed that in those 10 lines derived from female probands, the expression of a further 67 transcripts was arithmetically increased in every line and significantly increased overall and a further 332 transcripts was arithmetically decreased in every cell line and significantly decreased. Conversely, in cell lines from the six male probands, the expression of an additional 212 was arithmetically increased in every line and significantly increased overall and a further 332 transcripts was arithmetically decreased in every cell line. We conclude that lymphoblast cell lines derived from subjects with PD have significant, partially sex dependent changes in gene transcription. Further studies are necessary to correlate these changes in these hemopoetically derived cells with those changes postulated to occur in the CNS in association with PD.

Lymphocytes - metabolism

Cell Line

Diagnostic and Statistical Manual of Mental Disorders

Oligonucleotide Array Sequence Analysis

Humans

Middle Aged

Male

Gene Expression Profiling

Sex Characteristics

Adult

Female

Interview, Psychological

Panic Disorder - genetics

Details

Title: Subtitle: Transcriptional profiling of lymphoblast lines from subjects with panic disorder
Creators: Robert A Philibert - Department of Psychiatry, The University of Iowa, Iowa City, Iowa 52242, USA. robert-philibert@uiowa.edu
Raymond Crowe
Gi-Yung Ryu
Jae-Geun Yoon
Dianna Secrest
Harinder Sandhu
Anup Madan
Resource Type: Journal article
Publication Details: American journal of medical genetics. Part B, Neuropsychiatric genetics, Vol.144B(5), pp.674-682
DOI: 10.1002/ajmg.b.30502
PMID: 17342723
NLM abbreviation: Am J Med Genet B Neuropsychiatr Genet
ISSN: 1552-4841
eISSN: 1552-485X
Publisher: United States
Grant note: K08MH064714 / NIMH NIH HHS R01AI053264 / NIAID NIH HHS R01DA015789 / NIDA NIH HHS
Language: English
Date published: 07/05/2007
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Psychiatry; ICTS - Biomedical Informatics; Iowa Neuroscience Institute
Record Identifier: 9984003943702771

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