Journal article
Transcriptional regulation of estrogen receptor in breast carcinomas
Molecular and cellular biology, Vol.15(4), pp.2191-2196
04/1995
DOI: 10.1128/MCB.15.4.2191
PMCID: PMC230447
PMID: 7891714
Abstract
An important transcriptional regulatory element of the estrogen receptor (ER) gene that binds a protein expressed in ER-positive breast carcinomas has been identified. Using a transient expression assay, we identified a 75-bp region of the 5' untranslated leader of the ER gene which augments expression from the ER promoter. This region contains two binding sites for a protein, estrogen receptor factor 1 (ERF-1), which is expressed in ER-positive breast carcinomas. A concatenated ERF-1 binding site probe identified a 30,000-Da protein. Low-level ERF-1 expression was detected in normal human mammary epithelial cells. Abundant ERF-1 expression was also found in endometrial carcinoma cell lines that express the ER-positive phenotype. These results indicate that ERF-1 expression represents a common mechanism of ER regulation in hormonally responsive carcinomas.
Details
- Title: Subtitle
- Transcriptional regulation of estrogen receptor in breast carcinomas
- Creators
- Ellen C deConinck - Stanford UniversityLisa A McPherson - Stanford UniversityRonald J Weigel - Stanford University
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.15(4), pp.2191-2196
- DOI
- 10.1128/MCB.15.4.2191
- PMID
- 7891714
- PMCID
- PMC230447
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Grant note
- 1R29CA63251-01 / NCI NIH HHS
- Language
- English
- Date published
- 04/1995
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology; Surgery; Biochemistry and Molecular Biology
- Record Identifier
- 9984284331202771
Metrics
7 Record Views