Journal article
Transcriptome Analyses at Specific Plastochrons Reveal Timing and Involvement of Phytosulfokine in Maize Vegetative Phase Change
Plant science (Limerick), Vol.350, 112317
01/2025
DOI: 10.1016/j.plantsci.2024.112317
PMID: 39536951
Abstract
Successive developmental stages of representative early and late juvenile, transition, and adult maize leaves were compared using machine-learning-aided analyses of gene expression patterns to characterize vegetative phase change (VPC), including identification of the timing of this developmental transition in maize. We used t-SNE to organize 32 leaf samples into 9 groups with similar patterns of gene expression. oposSOM yielded clusters of co-expressed genes from key developmental stages. TO-GCN supported a sequence of events in maize in which germination-associated ROS triggers a JA response, both relieving oxidative stress and inducing miR156 production, which in turn spurs juvenility. Patterns of expression of MIR395, which regulates sulfur assimilation, led to the hypothesis that phytosulfokine, a sulfated peptide, is involved in the transition to adult patterns of differentiation.
•timing of vegetative phase change in maize was more precisely identified•miR156 production in juvenile leaf primordia follows ROS-induced high JA levels•Phytosulfokine plays a role in adult leaf development
Details
- Title: Subtitle
- Transcriptome Analyses at Specific Plastochrons Reveal Timing and Involvement of Phytosulfokine in Maize Vegetative Phase Change
- Creators
- Krista Osadchuk - University of IowaBen Beydler - University of IowaChi-Lien Cheng - University of IowaErin Irish - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Plant science (Limerick), Vol.350, 112317
- Publisher
- Elsevier B.V; CLARE
- DOI
- 10.1016/j.plantsci.2024.112317
- PMID
- 39536951
- ISSN
- 0168-9452
- eISSN
- 1873-2259
- Grant note
- NSF: 0820562 U. Iowa Avis Cone fellowships
This work was funded by NSF 0820562, with U. Iowa Avis Cone fellowships to K.O. and B.B. The authors thank Josh Strable and two anonymous reviewers for their helpful comments.
- Language
- English
- Electronic publication date
- 11/11/2024
- Date published
- 01/2025
- Academic Unit
- Biology; University College Courses
- Record Identifier
- 9984748159002771
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