Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer

Nicholas Borcherding; Nicholas Bormann; David Kusner; Ryan Kolb; Weizhou Zhang

doi:10.1016/j.gdata.2015.04.008

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Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer

Journal article

Open access

Peer reviewed

Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer

Nicholas Borcherding, Nicholas Bormann, David Kusner, Ryan Kolb and Weizhou Zhang

Genomics data, Vol.4(C), pp.119-122

06/2015

DOI: 10.1016/j.gdata.2015.04.008

PMCID: PMC4493742

PMID: 26167451

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Abstract

Breast cancer is the leading cause of cancer-related mortality for females worldwide [1]. Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigenic capacity between different mammary epithelial cell populations in an MMTV-ErbB2 mouse model. In order to greater understand how different mammary epithelial cells influence the tumorigenic capacity in ErbB2-induced breast cancer, we transplanted different cell populations from pre-neoplastic MMTV-ErbB2 female mice into recipient mice for tumorigenic study. We found that different mammary epithelial cells bear different tumorigenic potentials even when induced by the same ErbB2 proto-oncogene. To understand the difference in tumors formed from different epithelial cells, we performed gene expression profiling using these tumors (GSE64487). Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR). Here we provide further details on the experimental methods and microarray analysis. This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).

Breast Cancer

Tumor-initiating cells

Myoepithelial cells

Luminal progenitors

Mammary stem cells

Details

Title: Subtitle: Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
Creators: Nicholas Borcherding - Department of Pathology, United States
Nicholas Bormann - Carver College of Medicine, University of Iowa, Iowa City, IA 52242-1109, United States
David Kusner - University of Iowa, Internal Medicine
Ryan Kolb - Department of Pathology, United States
Weizhou Zhang - Department of Pathology, United States
Resource Type: Journal article
Publication Details: Genomics data, Vol.4(C), pp.119-122
DOI: 10.1016/j.gdata.2015.04.008
PMID: 26167451
PMCID: PMC4493742
NLM abbreviation: Genom Data
ISSN: 2213-5960
eISSN: 2213-5960
Publisher: Elsevier Inc
Grant note: DOI: 10.13039/100000002, name: NIH, award: K99/R00 CA158055; DOI: 10.13039/100000002, name: NIH, award: T32 GM007337; DOI: 10.13039/100000002, name: NIH, award: T32 AI007260; name: Department Startup Grant and Seed Grant; name: Breast Cancer Research Grant; name: Holden Comprehensive Cancer Center, University of Iowa Carver College of Medicine
Language: English
Date published: 06/2015
Academic Unit: Dermatology; Radiation Oncology; Internal Medicine
Record Identifier: 9984083270502771

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