Journal article
Transcriptome profiling of subepithelial PDGFR alpha cells in colonic mucosa reveals several cell-selective markers
PloS one, Vol.17(5), pp.e0261743-e0261743
01/01/2022
DOI: 10.1371/journal.pone.0261743
PMCID: PMC9106222
PMID: 35560163
Abstract
Subepithelial platelet-derived growth factor receptor alpha (PDGFR alpha)(+) cells found in the colonic mucosal tissue come in close contact with epithelial cells, immune cells, neurons, capillaries, and lymphatic networks. Mucosal subepithelial PDGFR alpha(+) cells (MuP alpha C) are important regulators in various intestinal diseases including fibrosis and inflammation. However, the transcriptome of MuP alpha C has not yet been elucidated. Using Pdgfra-eGFP mice and flow cytometry, we isolated colonic MuP alpha C and obtained their transcriptome data. In analyzing the transcriptome, we identified three novel, and selectively expressed, markers (Adamdec1, Fin1, and Col6a4) found in MuP alpha C. In addition, we identified a unique set of MuP alpha C-enriched genetic signatures including groups of growth factors, transcription factors, gap junction proteins, extracellular proteins, receptors, cytokines, protein kinases, phosphatases, and peptidases. These selective groups of genetic signatures are linked to the unique cellular identity and function of MuP alpha C. Furthermore, we have added this MuP alpha C transcriptome data to our Smooth Muscle Genome Browser that contains the transcriptome data of jejunal and colonic smooth muscle cells (SMC), interstitial cells of Cajal (ICC), and smooth muscle resident PDGFR alpha(+) cells: (). This online resource provides a comprehensive reference of all currently known genetic transcripts expressed in primary MuP alpha C in the colon along with smooth muscle resident PDGFR alpha cells, SMC, and ICC in the murine colon and jejunum.
Details
- Title: Subtitle
- Transcriptome profiling of subepithelial PDGFR alpha cells in colonic mucosa reveals several cell-selective markers
- Creators
- Se Eun Ha - University of Nevada, RenoByungchang Jin - University of Nevada, RenoBrian G. Jorgensen - University of Nevada, RenoHannah Zogg - University of Nevada, RenoLai Wei - University of Nevada, RenoRajan Singh - University of Nevada, RenoChanjae Park - University of Nevada, RenoMasaaki Kurahashi - University of IowaSei Kim - University of Nevada, RenoGain Baek - University of Nevada, RenoSandra M. Poudrier - University of Nevada, RenoMoon Young Lee - Wonkwang UniversityKenton M. Sanders - University of Nevada, RenoSeungil Ro - University of Nevada, Reno
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.17(5), pp.e0261743-e0261743
- DOI
- 10.1371/journal.pone.0261743
- PMID
- 35560163
- PMCID
- PMC9106222
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library Science
- Number of pages
- 24
- Grant note
- DK094886; DK103055; DK091336; P01DK041315 / National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 01/01/2022
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984359932302771
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