Journal article
Transcutaneous DNA immunization following waxing-based hair depilation
Journal of controlled release, Vol.157(1), pp.94-102
2012
DOI: 10.1016/j.jconrel.2011.08.038
PMCID: PMC3260413
PMID: 21907253
Abstract
Transcutaneous DNA immunization is an attractive immunization approach. Previously, we reported that transcutaneous immunization by applying plasmid DNA onto a skin area wherein the hair follicles had been induced into growth stage by ‘cold’ waxing-based hair plucking significantly enhanced the resultant immune responses. In the present study, using a plasmid that encodes the
Bacillus anthracis protective antigen (PA63) gene fragment, it was shown that the anti-PA63 antibody responses induced by applying the plasmid onto a skin area where the hair was plucked by ‘warm’ waxing were significantly stronger than by ‘cold’ waxing, very likely because the ‘warm’ waxing-based hair depilation significantly i) enhanced the uptake (or retention) of the plasmid in the application area and ii) enhanced the expression of the transfected gene in the follicular and interfollicular epidermis in the skin. The antibody response induced by transcutaneous DNA immunization was hair cycle dependent, because the plasmid needed to be applied within 5
days after the hair plucking to induce a strong antibody response. The antibody responses were not affected by whether the expressed PA63 protein, as an antigen, was secreted or cell surface bound. Finally, this strategy of enhancing the immune responses induced by transcutaneous DNA immunization following ‘warm’ waxing-based hair depilation was not limited to the PA63 as an antigen, because immunization with a plasmid that encodes the HIV-1
env gp160 gene induced a strong anti-gp160 response as well. Transcutaneous DNA immunization by modifying the hair follicle cycle may hold a great promise in inducing strong and functional immune responses.
Transcutaneous DNA immunization following 'warm' waxing-based hair depilation induced a strong antibody response (A) and enhanced the expression of the transfected gene (B).
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Details
- Title: Subtitle
- Transcutaneous DNA immunization following waxing-based hair depilation
- Creators
- Brian R Sloat - The University of Texas at Austin, College of Pharmacy, Pharmaceutics Division, Austin, Texas, 78712, United StatesKaoru Kiguchi - The University of Texas at Austin, College of Pharmacy, Pharmacology & Toxicology Division, Austin, Texas, 78712, United StatesGang Xiao - The University of Texas at Austin, College of Pharmacy, Pharmaceutics Division, Austin, Texas, 78712, United StatesJohn DiGiovanni - The University of Texas at Austin, College of Pharmacy, Pharmacology & Toxicology Division, Austin, Texas, 78712, United StatesWendy Maury - University of Iowa, Carver College of Medicine, Department of Microbiology, Iowa City, IA 52242, United StatesZhengrong Cui - The University of Texas at Austin, College of Pharmacy, Pharmaceutics Division, Austin, Texas, 78712, United States
- Resource Type
- Journal article
- Publication Details
- Journal of controlled release, Vol.157(1), pp.94-102
- DOI
- 10.1016/j.jconrel.2011.08.038
- PMID
- 21907253
- PMCID
- PMC3260413
- NLM abbreviation
- J Control Release
- ISSN
- 0168-3659
- eISSN
- 1873-4995
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2012
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083288002771
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