Journal article
Transdifferentiation of tadpole pancreatic acinar cells to duct cells mediated by Notch and stromelysin-3
Developmental biology, Vol.351(2), pp.311-317
03/15/2011
DOI: 10.1016/j.ydbio.2010.12.020
PMCID: PMC3394455
PMID: 21194527
Abstract
The tadpole pancreas has differentiated acinar cells but an underdeveloped ductal system. At the climax of metamorphosis thyroid hormone (TH) induces the tadpole acinar cells to dedifferentiate to a progenitor state. After metamorphosis is complete the exocrine pancreas redifferentiates in the growing frog forming a typical vertebrate pancreas including a complex ductal system. A micro array analysis found that TH up regulates stromelysin 3 (ST3, matrix metalloproteinase 11) in the exocrine pancreas at metamorphic climax. Transgenic tadpoles were prepared with an elastase promoter driving either the ST3 gene or the constitutively active form of Notch (IC). Expression of the transgenes was controlled by the tetracycline system. A few days after either of these transgenes is activated by doxycycline the pancreatic acinar cells turn into duct-like cells. This transdetermination occurs without cell division since both acinar and ductal markers can be visualized transiently in the same cell. We propose that remodeling of the tadpole acinar cells is initiated when ST3 is up regulated by TH. Stromelysin-3 then cleaves and activates Notch. (C) 2010 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Transdifferentiation of tadpole pancreatic acinar cells to duct cells mediated by Notch and stromelysin-3
- Creators
- Sandeep Mukhi - Carnegie Institution for ScienceDonald D. Brown - Carnegie Inst, Baltimore, MD 21218 USA
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.351(2), pp.311-317
- DOI
- 10.1016/j.ydbio.2010.12.020
- PMID
- 21194527
- PMCID
- PMC3394455
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- R01GM022395 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA G. Harold and Leila Y. Mathers Charitable Trust
- Language
- English
- Date published
- 03/15/2011
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984361573802771
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