Journal article
Transdifferentiation of vocal-fold stellate cells and all-trans retinol-induced deactivation
Cell and tissue research, Vol.322(3), pp.417-424
12/01/2005
DOI: 10.1007/s00441-005-0028-9
PMID: 16047162
Abstract
The maculae flavae of the human vocal folds include dense extracellular matrices and compacted cells with a stellate morphology. These vocal-fold stellate cells are thought to participate in the metabolism of extracellular matrices essential in maintaining vocal-fold viscoelasticity required for phonation. We have isolated and cultured these new cells and have tested the hypothesis that they maintain a distinct cellular and biochemical phenotype. We have compared proliferation rates, changes on immunophenotype, and intracellular lipid and vitamin A storage. Vocal-fold stellate cells undergo culture-induced transdifferentiation to a myofibroblast-like phenotype with an altered phenotype resembling, but not identical to, activated hepatic and pancreatic stellate cells. Our results reveal that these cells are capable of responding to exogenous all-trans retinol in culture. Exposure to this synthetic co-factor causes deactivation characterized by decreased proliferation, loss of the activated stellate cell marker, alpha-smooth muscle actin, and restoration of intracellular lipid and vitamin A metabolite storage. These data establish a new and distinct cellular target for future investigations of the viscoelastic properties of the vocal-fold mucosa during normal phonation, aging, vocal-fold scarring, laryngeal fibrosis, and myofibroblastoma.
Details
- Title: Subtitle
- Transdifferentiation of vocal-fold stellate cells and all-trans retinol-induced deactivation
- Creators
- Tannin J Fuja - University of IowaMegan N Probst-Fuja - University of IowaIngo R Titze - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cell and tissue research, Vol.322(3), pp.417-424
- DOI
- 10.1007/s00441-005-0028-9
- PMID
- 16047162
- ISSN
- 0302-766X
- eISSN
- 1432-0878
- Number of pages
- 8
- Language
- English
- Date published
- 12/01/2005
- Academic Unit
- School of Music; Communication Sciences and Disorders
- Record Identifier
- 9984719568502771
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