Transducin Partners Outside the Phototransduction Pathway

Dhiraj Srivastava; Ravi P Yadav; Shivangi M Inamdar; Zhen Huang; Maxim Sokolov; Kimberly Boyd; Nikolai O Artemyev

doi:10.3389/fncel.2020.589494

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Transducin Partners Outside the Phototransduction Pathway

Journal article

Open access

Peer reviewed

Transducin Partners Outside the Phototransduction Pathway

Dhiraj Srivastava, Ravi P Yadav, Shivangi M Inamdar, Zhen Huang, Maxim Sokolov, Kimberly Boyd and Nikolai O Artemyev

Frontiers in cellular neuroscience, Vol.14, pp.589494-589494

10/14/2020

DOI: 10.3389/fncel.2020.589494

PMCID: PMC7591391

PMID: 33173469

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Abstract

Transducin mediates signal transduction in a classical G protein-coupled receptor (GPCR) phototransduction cascade. Interactions of transducin with the receptor and the effector molecules had been extensively investigated and are currently defined at the atomic level. However, partners and functions of rod transducin α (Gα t 1 ) and βγ (Gβ 1 γ 1 ) outside the visual pathway are not well-understood. In particular, light-induced redistribution of rod transducin from the outer segment to the inner segment and synaptic terminal (IS/ST) allows Gα t1 and/or Gβ 1 γ 1 to modulate synaptic transmission from rods to rod bipolar cells (RBCs). Protein-protein interactions underlying this modulation are largely unknown. We discuss known interactors of transducin in the rod IS/ST compartment and potential pathways leading to the synaptic effects of light-dispersed Gα t1 and Gβ 1 γ 1 . Furthermore, we show that a prominent non-GPCR guanine nucleotide exchange factor (GEF) and a chaperone of Gα subunits, resistance to inhibitors of cholinesterase 8A (Ric-8A) protein, is expressed throughout the retina including photoreceptor cells. Recent structures of Ric-8A alone and in complexes with Gα subunits have illuminated the structural underpinnings of the Ric-8A activities. We generated a mouse model with conditional knockout of Ric-8A in rods in order to begin defining the functional roles of the protein in rod photoreceptors and the retina. Our analysis suggests that Ric-8A is not an obligate chaperone of Gα t1 . Further research is needed to investigate probable roles of Ric-8A as a GEF, trafficking chaperone, or a mediator of the synaptic effects of Gα t1 .

Neuroscience

chaperone

retina

Ric-8A

GPCR

GEF

transducin

G protein

Details

Title: Subtitle: Transducin Partners Outside the Phototransduction Pathway
Creators: Dhiraj Srivastava - Department of Molecular Physiology and Biophysics, The University of Iowa Carver College of Medicine
Ravi P Yadav - Department of Molecular Physiology and Biophysics, The University of Iowa Carver College of Medicine
Shivangi M Inamdar - Department of Molecular Physiology and Biophysics, The University of Iowa Carver College of Medicine
Zhen Huang - Department of Neurology and Neuroscience, University of Wisconsin-Madison
Maxim Sokolov - Department of Ophthalmology, Biochemistry and Neuroscience, West Virginia University
Kimberly Boyd - Department of Molecular Physiology and Biophysics, The University of Iowa Carver College of Medicine
Nikolai O Artemyev - Department of Molecular Physiology and Biophysics, The University of Iowa Carver College of Medicine
Resource Type: Journal article
Publication Details: Frontiers in cellular neuroscience, Vol.14, pp.589494-589494
DOI: 10.3389/fncel.2020.589494
PMID: 33173469
PMCID: PMC7591391
NLM abbreviation: Front Cell Neurosci
ISSN: 1662-5102
eISSN: 1662-5102
Publisher: Frontiers Media S.A
Grant note: National Institutes of Health
Language: English
Date published: 10/14/2020
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984070422402771

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