Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells

Anurima Majumder; Johan Pahlberg; Kimberly K Boyd; Vasily Kerov; Saravanan Kolandaivelu; Visvanathan Ramamurthy; Alapakkam P Sampath; Nikolai O Artemyev

doi:10.1073/pnas.1222666110

Back

Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells

Journal article

Open access

Peer reviewed

Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells

Anurima Majumder, Johan Pahlberg, Kimberly K Boyd, Vasily Kerov, Saravanan Kolandaivelu, Visvanathan Ramamurthy, Alapakkam P Sampath and Nikolai O Artemyev

Proceedings of the National Academy of Sciences - PNAS, Vol.110(30), pp.12468-12473

07/23/2013

DOI: 10.1073/pnas.1222666110

PMCID: PMC3725049

PMID: 23836670

Files and links (1)

url

https://doi.org/10.1073/pnas.1222666110View

Published (Version of record) Open Access

Abstract

In rod photoreceptors, several phototransduction components display light-dependent translocation between cellular compartments. Notably, the G protein transducin translocates from rod outer segments to inner segments/spherules in bright light, but the functional consequences of translocation remain unclear. We generated transgenic mice where light-induced transducin translocation is impaired. These mice exhibited slow photoreceptor degeneration, which was prevented if they were dark-reared. Physiological recordings showed that control and transgenic rods and rod bipolar cells displayed similar sensitivity in darkness. After bright light exposure, control rods were more strongly desensitized than transgenic rods. However, in rod bipolar cells, this effect was reversed; transgenic rod bipolar cells were more strongly desensitized than control. This sensitivity reversal indicates that transducin translocation in rods enhances signaling to rod bipolar cells. The enhancement could not be explained by modulation of inner segment conductances or the voltage sensitivity of the synaptic Ca 2+ current, suggesting interactions of transducin with the synaptic machinery.

Biological Sciences

adaptation

SNARE complex

presynaptic modulation

retina

palmitoylation

Details

Title: Subtitle: Transducin translocation contributes to rod survival and enhances synaptic transmission from rods to rod bipolar cells
Creators: Anurima Majumder - Departments of
Johan Pahlberg - Zilkha Neurogenetic Institute, Department of Physiology and Biophysics
Kimberly K Boyd - Departments of
Vasily Kerov - Departments of
Saravanan Kolandaivelu - Department of Ophthalmology and Biochemistry
Visvanathan Ramamurthy - Department of Ophthalmology and Biochemistry
Alapakkam P Sampath - Zilkha Neurogenetic Institute, Department of Physiology and Biophysics
Nikolai O Artemyev - Departments of
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.110(30), pp.12468-12473
DOI: 10.1073/pnas.1222666110
PMID: 23836670
PMCID: PMC3725049
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Alternative title: G-protein control of synaptic transmission in rods
Language: English
Date published: 07/23/2013
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984025450902771

Metrics

18 Record Views

38 Times Cited - Web of Science