Transendothelial chemotaxis of human alpha/beta and gamma/delta T lymphocytes to chemokines

Stephen J Roth; Thomas G Diacovo; Michael B Brenner; Jean-Pierre Rosat; Janet Buccola; Craig T Morita; Timothy A Springer

doi:10.1002/(SICI)1521-4141(199801)28:01<104::AID-IMMU104>3.0.CO;2-F

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Transendothelial chemotaxis of human alpha/beta and gamma/delta T lymphocytes to chemokines

Journal article

Peer reviewed

Transendothelial chemotaxis of human alpha/beta and gamma/delta T lymphocytes to chemokines

Stephen J Roth, Thomas G Diacovo, Michael B Brenner, Jean-Pierre Rosat, Janet Buccola, Craig T Morita and Timothy A Springer

European journal of immunology, Vol.28(1), pp.104-113

01/1998

DOI: 10.1002/(SICI)1521-4141(199801)28:01<104::AID-IMMU104>3.0.CO;2-F

PMID: 9485190

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Abstract

Two subpopulations of human T lymphocytes expressing different antigen receptors, alpha/beta and gamma/delta, emigrate into inflamed tissues in distinctive patterns. We compared the transmigration of alpha/beta and gamma/delta T cells to C-C and C-X-C chemokines using an in vitro transendothelial chemotaxis assay. The C-C chemokines monocyte chemoattractant protein (MCP)-1, RANTES, macrophage inflammatory protein (MIP)-1alpha and MIP-1beta stimulated similar, dose-dependent chemotaxis of purified gamma/delta T cells, whereas MCP-1, RANTES, and MIP-1alpha produced greater chemotaxis of purified alpha/beta T cells than MIP-1beta. In contrast, the C-X-C chemokines interleukin (IL)-8 and interferon-gamma inducible protein-10 (IP-10) did not promote chemotaxis of either alpha/beta or gamma/delta T cells. Three gamma/delta T cell clones with differing CD4 and CD8 phenotypes also migrated exclusively to C-C chemokines. Phenotypic analysis of mononuclear cells that transmigrated from an input population of unfractionated peripheral blood mononuclear cells confirmed the results with purified gamma/delta T cells. Our data demonstrate that human peripheral blood alpha/beta and gamma/delta T cells can transmigrate to MCP-1, RANTES, MIP-1alpha, and MIP-1beta, and suggest that both T lymphocyte subpopulations share the capacity to emigrate in response to C-C chemokines during inflammation.

Inflammation

Chemokine CCL4

Chemokine CCL3

Dose-Response Relationship, Immunologic

T-Lymphocyte Subsets - cytology

Chemokines, CXC

Humans

Receptors, Antigen, T-Cell, alpha-beta - analysis

Recombinant Proteins - pharmacology

Chemokine CXCL10

Chemotactic Factors - pharmacology

Chemokine CCL5 - pharmacology

Receptors, Antigen, T-Cell, gamma-delta - analysis

Chemokine CCL2 - pharmacology

Chemotaxis, Leukocyte - drug effects

T-Lymphocyte Subsets - drug effects

Chemokines - pharmacology

Endothelium, Vascular

Macrophage Inflammatory Proteins - pharmacology

Details

Title: Subtitle: Transendothelial chemotaxis of human alpha/beta and gamma/delta T lymphocytes to chemokines
Creators: Stephen J Roth - Department of Cardiology, Children's Hospital, Boston, USA
Thomas G Diacovo
Michael B Brenner
Jean-Pierre Rosat
Janet Buccola
Craig T Morita
Timothy A Springer
Resource Type: Journal article
Publication Details: European journal of immunology, Vol.28(1), pp.104-113
DOI: 10.1002/(SICI)1521-4141(199801)28:01<104::AID-IMMU104>3.0.CO;2-F
PMID: 9485190
ISSN: 0014-2980
eISSN: 1521-4141
Grant note: HL03361 / NHLBI NIH HHS HL48675 / NHLBI NIH HHS HL02776 / NHLBI NIH HHS
Language: English
Date published: 01/1998
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094751802771

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