Journal article
Transferrin receptor synthesis is an early event in B cell activation
The Journal of immunology (1950), Vol.143(3), pp.787-792
08/01/1989
DOI: 10.4049/jimmunol.143.3.787
PMID: 2473110
Abstract
Purified, small resting mouse B cells appear to express low levels of transferrin receptor (TfR) as detected by flow cytometry. Moreover, when such cells are stimulated with LPS or F(ab')2 anti-mu, they show increased expression of TfR as early as 4 h after activation when the cells are at the boundary of G0 and G1 phase by cell cycle analysis. Cells treated with anti-mu increased TfR expression gradually, reaching a plateau after 46 h, whereas cells with LPS reached their plateau by 12 h. The kinetics of induction of increased TfR expression was similar for both small and large B cells. Inhibition of protein synthesis with anisomycin or inhibition of transcription with actinomycin-D blocked the increased expression of TfR at 4 h. Northern blot analysis showed a marked increase of TfR mRNA at 2 and 4 h with either anti-mu or LPS which was then followed by an apparent decline at 16 h. These findings together with other recent studies justify reevaluation of the generally accepted models placing TfR induction late in the G1 phase of the lymphocyte activation sequence. They support the concept that in the B lymphocyte the TfR gene is selectively transcribed earlier than previously thought, before the general increase in RNA synthesis.
Details
- Title: Subtitle
- Transferrin receptor synthesis is an early event in B cell activation
- Creators
- Jacobo Futran - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242John D KempElizabeth H FieldAnuradha VoraRobert F Ashman
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.143(3), pp.787-792
- DOI
- 10.4049/jimmunol.143.3.787
- PMID
- 2473110
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AI22630 / NIAID NIH HHS
- Language
- English
- Date published
- 08/01/1989
- Academic Unit
- Pathology; Immunology; Internal Medicine
- Record Identifier
- 9984094586902771
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