Journal article
Transformation of Mollicutes with Single-Stranded Tn4001 DNA
Plasmid, Vol.32(1), pp.85-88
07/1994
DOI: 10.1006/plas.1994.1048
PMID: 7991677
Abstract
Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA.
Details
- Title: Subtitle
- Transformation of Mollicutes with Single-Stranded Tn4001 DNA
- Creators
- Paul A. Kapke - Iowa State UniversityKevin L. Knudtson - Iowa State UniversityF.Chris Minion - Iowa State University
- Resource Type
- Journal article
- Publication Details
- Plasmid, Vol.32(1), pp.85-88
- Publisher
- Elsevier Inc
- DOI
- 10.1006/plas.1994.1048
- PMID
- 7991677
- ISSN
- 0147-619X
- eISSN
- 1095-9890
- Language
- English
- Date published
- 07/1994
- Academic Unit
- Iowa Institute of Human Genetics
- Record Identifier
- 9984627286802771
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