Journal article
Transformation of Primary Chick Embryo Fibroblasts by Marek's Disease Virus
Virology (New York, N.Y.), Vol.239(1), pp.20-35
12/08/1997
DOI: 10.1006/viro.1997.8854
PMID: 9426443
Abstract
Marek's disease virus (MDV) is an alphaherpesvirus, which can mediate the malignant transformation of lymphocytes to form lymphomas in chickens. In this study, we demonstrate that MDV can transform primary chick embryo fibroblasts (CEF). The cell line derived from primary CEF infected with the GA strain of MDV was called CEM(MDV). The fibroblast nature of CEM(MDV)was verified by absence of cytokeratin type II. The CEM(MDV)phenotype differed from either primary CEF or MDV-infected CEF. CEM(MDV)were extensively vacuolated, with unusual multilamellar structures in the cytoplasm. The nuclei were considerably larger than those in primary CEF and were uniformly positive for proliferating cell nuclear antigen. The cell line was subcultured for more than 10 generations; however, CEM(MDV)did not support a fully productive MDV infection, because complete nucleocapsids were not detected and infectivity assays showed that the cell line produced no infectious virus. PCR analyses demonstrated that this cell line carried both polypeptide 38 (pp38) and Meq DNA, MDV-specific genes associated with transformation. In addition, examination by laser scanning confocal microscopy revealed that CEM(MDV)constitutively produced MDV MEQ protein in nuclei and pp38 as well as glycoprotein B in the cytoplasm and on the plasma membrane. Growth in soft agar assay demonstrated that CEM(MDV)formed colonies, similar to HeLa and human melanoma cells. Retroviral insertion was not detected in DNA from the CEM(MDV)line.
Details
- Title: Subtitle
- Transformation of Primary Chick Embryo Fibroblasts by Marek's Disease Virus
- Creators
- Chantanee BuranathaiJose RodriguezCharles Grose
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.239(1), pp.20-35
- DOI
- 10.1006/viro.1997.8854
- PMID
- 9426443
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 12/08/1997
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093375402771
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