Transforming growth factor Beta 3 is required for excisional wound repair in vivo

Mark Le; Rachelle Naridze; Jasmine Morrison; Leah C Biggs; Lindsey Rhea; Brian C Schutte; Vesa Kaartinen; Martine Dunnwald

doi:10.1371/journal.pone.0048040

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Transforming growth factor Beta 3 is required for excisional wound repair in vivo

Journal article

Open access

Peer reviewed

Transforming growth factor Beta 3 is required for excisional wound repair in vivo

Mark Le, Rachelle Naridze, Jasmine Morrison, Leah C Biggs, Lindsey Rhea, Brian C Schutte, Vesa Kaartinen and Martine Dunnwald

PloS one, Vol.7(10), pp.e48040-e48040

2012

DOI: 10.1371/journal.pone.0048040

PMCID: PMC3482237

PMID: 23110169

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Abstract

Wound healing is a complex process that relies on proper levels of cytokines and growth factors to successfully repair the tissue. Of particular interest are the members of the transforming growth factor family. There are three TGF-ß isoforms-TGF- ß 1, 2, and 3, each isoform showing a unique expression pattern, suggesting that they each play a distinct function during development and repair. Previous studies reported an exclusive role for TGF-ß 3 in orofacial development and a potent anti-scarring effect. However, the role of TGF- ß 3 in excisional wound healing and keratinocyte migration remains poorly understood. We tested the effect of TGF-ß 3 levels on excisional cutaneous wounds in the adult mouse by directly injecting recombinant TGF-ß 3 or neutralizing antibody against TGF-ß 3 (NAB) in the wounds. Our results demonstrate that TGF-ß 3 does not promote epithelialization. However, TGF-ß 3 is necessary for wound closure as wounds injected with neutralizing antibody against TGF-ß 3 showed increased epidermal volume and proliferation in conjunction with a delay in keratinocyte migration. Wild type keratinocytes treated with NAB and Tgfb3-deficient keratinocytes closed an in vitro scratch wound with no delay, suggesting that our in vivo observations likely result from a paracrine effect.

Immunohistochemistry

Wound Healing

Transforming Growth Factor beta3 - genetics

Male

Transforming Growth Factor beta3 - metabolism

Cell Movement - genetics

Cicatrix - metabolism

Antibodies, Neutralizing - immunology

Time Factors

Transforming Growth Factor beta3 - immunology

Female

Recombinant Proteins - metabolism

Epithelium - drug effects

Epithelium - pathology

Epithelium - metabolism

Mice, Inbred C57BL

Cells, Cultured

Antibodies, Neutralizing - pharmacology

Mice, Transgenic

Recombinant Proteins - pharmacology

Cicatrix - genetics

Cell Movement - drug effects

Keratinocytes - pathology

Animals

Keratinocytes - drug effects

Keratinocytes - metabolism

Cell Proliferation - drug effects

Mice

Details

Title: Subtitle: Transforming growth factor Beta 3 is required for excisional wound repair in vivo
Creators: Mark Le - Department of Pediatrics, Carver College of Medicine, The University of Iowa, Iowa City, Iowa, United States of America
Rachelle Naridze
Jasmine Morrison
Leah C Biggs
Lindsey Rhea
Brian C Schutte
Vesa Kaartinen
Martine Dunnwald
Resource Type: Journal article
Publication Details: PloS one, Vol.7(10), pp.e48040-e48040
DOI: 10.1371/journal.pone.0048040
PMID: 23110169
PMCID: PMC3482237
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: P50 DE016215 / NIDCR NIH HHS R03 AR055313 / NIAMS NIH HHS AR055313 / NIAMS NIH HHS DE16215 / NIDCR NIH HHS
Language: English
Date published: 2012
Academic Unit: Anatomy and Cell Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984025338502771

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