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Transgenesis procedures in Xenopus
Journal article   Open access   Peer reviewed

Transgenesis procedures in Xenopus

Albert Chesneau, Laurent M Sachs, Norin Chai, Yonglong Chen, Louis Du Pasquier, Jana Loeber, Nicolas Pollet, Michael Reilly, Daniel L Weeks and Odile J Bronchain
Biology of the cell, Vol.100(9), pp.503-529
09/2008
DOI: 10.1042/BC20070148
PMCID: PMC2967756
PMID: 18699776
url
https://www.ncbi.nlm.nih.gov/pmc/articles/2967756View
Open Access

Abstract

Stable integration of foreign DNA into the frog genome has been the purpose of several studies aimed at generating transgenic animals or producing mutations of endogenous genes. Inserting DNA into a host genome can be achieved in a number of ways. In Xenopus, different strategies have been developed which exhibit specific molecular and technical features. Although several of these technologies were also applied in various model organizms, the attributes of each method have rarely been experimentally compared. Investigators are thus confronted with a difficult choice to discriminate which method would be best suited for their applications. To gain better understanding, a transgenesis workshop was organized by the X-omics consortium. Three procedures were assessed side-by-side, and the results obtained are used to illustrate this review. In addition, a number of reagents and tools have been set up for the purpose of gene expression and functional gene analyses. This not only improves the status of Xenopus as a powerful model for developmental studies, but also renders it suitable for sophisticated genetic approaches. Twenty years after the first reported transgenic Xenopus, we review the state of the art of transgenic research, focusing on the new perspectives in performing genetic studies in this species.
 Life Sciences Xenopus Gene Transfer Techniques Animals Animals, Genetically Modified Neurons and Cognition Genetic Techniques Genetic Vectors

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