Journal article
Transgenic mice expressing an inhibitory truncated form of p300 exhibit long-term memory deficits
Learning & memory (Cold Spring Harbor, N.Y.), Vol.14(9), pp.564-572
09/2007
DOI: 10.1101/lm.656907
PMCID: PMC1994075
PMID: 17761541
Abstract
The formation of many forms of long-term memory requires several molecular mechanisms including regulation of gene expression. The mechanisms directing transcription require not only activation of individual transcription factors but also recruitment of transcriptional coactivators. CBP and p300 are transcriptional coactivators that interact with a large number of transcription factors and regulate transcription through multiple mechanisms, including an intrinsic histone acetyltransferase (HAT) activity. HAT activity mediates acetylation of lysine residues on the amino-terminal tails of histone proteins, thereby increasing DNA accessibility for transcription factors to activate gene expression. CBP has been shown to play an important role in long-term memory formation. We have investigated whether p300 is also required for certain forms of memory. p300 shares a high degree of homology with CBP and has been shown to interact with transcription factors known to be critical for long-term memory formation. Here we demonstrate that conditional transgenic mice expressing an inhibitory truncated form of p300 (p300Δ1), which lacks the carboxy-terminal HAT and activation domains, have impaired long-term recognition memory and contextual fear memory. Thus, our study demonstrates that p300 is required for certain forms of memory and that the HAT and carboxy-terminal domains play a critical role.
Details
- Title: Subtitle
- Transgenic mice expressing an inhibitory truncated form of p300 exhibit long-term memory deficits
- Creators
- Ana M.M Oliveira - Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USAMarcelo A Wood - Department of Neurobiology and Behavior, Center for the Neurobiology of Learning and Memory, University of California, Irvine, California 92697, USAConor B McDonough - Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USATed Abel - Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
- Resource Type
- Journal article
- Publication Details
- Learning & memory (Cold Spring Harbor, N.Y.), Vol.14(9), pp.564-572
- Publisher
- Cold Spring Harbor Laboratory Press
- DOI
- 10.1101/lm.656907
- PMID
- 17761541
- PMCID
- PMC1994075
- ISSN
- 1072-0502
- eISSN
- 1549-5485
- Alternative title
- p300 and long-term memory
- Language
- English
- Date published
- 09/2007
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984065828902771
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