Journal article
Transglutaminase-2 facilitates extracellular vesicle-mediated establishment of the metastatic niche
Oncogenesis (New York, NY), Vol.9(2), pp.16-16
02/13/2020
DOI: 10.1038/s41389-020-0204-5
PMCID: PMC7018754
PMID: 32054828
Abstract
The ability of breast cancer cells to interconvert between epithelial and mesenchymal states contributes to their metastatic potential. As opposed to cell autonomous effects, the impact of epithelial-mesenchymal plasticity (EMP) on primary and metastatic tumor microenvironments remains poorly characterized. Herein we utilize global gene expression analyses to characterize a metastatic model of EMP as compared to their non-metastatic counterparts. Using this approach, we demonstrate that upregulation of the extracellular matrix crosslinking enzyme tissue transglutaminase-2 (TG2) is part of a novel gene signature that only emerges in metastatic cells that have undergone induction and reversion of epithelial-mesenchymal transition (EMT). Consistent with our model system, patient survival is diminished when primary tumors demonstrate enhanced levels of TG2 in conjunction with its substrate, fibronectin. Targeted depletion of TG2 inhibits metastasis, while overexpression of TG2 is sufficient to enhance this process. In addition to being present within cells, we demonstrate a robust increase in the amount of TG2 and crosslinked fibronectin present within extracellular vesicle (EV) fractions derived from metastatic breast cancer cells. Confocal microscopy of these EVs suggests that FN undergoes fibrillogenesis on their surface via a TG2 and Tensin1-dependent process. Upon in vivo administration, the ability of tumor-derived EVs to induce metastatic niche formation and enhance subsequent pulmonary tumor growth requires the presence and activity of TG2. Finally, we develop a novel 3D model of the metastatic niche to demonstrate that conditioning of pulmonary fibroblasts via pretreatment with tumor-derived EVs promotes subsequent growth of breast cancer cells in a TG2-dependent fashion. Overall, our studies illustrate a novel mechanism through which EMP contributes to metastatic niche development and distant metastasis via tumor-derived EVs containing aberrant levels of TG2 and fibrillar FN.
Details
- Title: Subtitle
- Transglutaminase-2 facilitates extracellular vesicle-mediated establishment of the metastatic niche
- Creators
- Aparna Shinde - Purdue University West LafayetteJuan Sebastian Paez - Purdue University West LafayetteSarah Libring - Purdue University West LafayetteKelsey Hopkins - Purdue University West LafayetteLuis Solorio - Purdue University West LafayetteMichael K. Wendt - Purdue University West Lafayette
- Resource Type
- Journal article
- Publication Details
- Oncogenesis (New York, NY), Vol.9(2), pp.16-16
- DOI
- 10.1038/s41389-020-0204-5
- PMID
- 32054828
- PMCID
- PMC7018754
- NLM abbreviation
- Oncogenesis
- ISSN
- 2157-9024
- eISSN
- 2157-9024
- Publisher
- Springer Nature
- Number of pages
- 12
- Grant note
- NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30CA023168 / Purdue Center for Cancer Research via an NCI Center grant R01CA207751; R01CA232589; R21AA026675; R00CA198929 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA RSG-CSM130259 / American Cancer Society
- Language
- English
- Date published
- 02/13/2020
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984460335102771
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