Journal article
Transient and stable transfection in the protozoan parasite Entamoeba invadens
Molecular and biochemical parasitology, Vol.184(1), pp.59-62
07/01/2012
DOI: 10.1016/j.molbiopara.2012.04.007
PMID: 22561071
Abstract
Entamoeba histolytica is an important human pathogen and a major health problem worldwide. Many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in E. histolytica. The reptile parasite Entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. However, functional studies using E. invadens have been limited by the lack of genetic tools in this species. Here, we report a new method for both transient and stable transfection of E. invadens. These new tools will greatly enhance research into Entamoeba development. (C) 2012 Elsevier B.V. All rights reserved.
Details
- Title: Subtitle
- Transient and stable transfection in the protozoan parasite Entamoeba invadens
- Creators
- Gretchen M. Ehrenkaufer - Stanford MedicineUpinder Singh - Stanford Medicine
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.184(1), pp.59-62
- DOI
- 10.1016/j.molbiopara.2012.04.007
- PMID
- 22561071
- NLM abbreviation
- Mol Biochem Parasitol
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Publisher
- Elsevier
- Number of pages
- 4
- Grant note
- AI088042; AI094887 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 07/01/2012
- Academic Unit
- Internal Medicine
- Record Identifier
- 9985244543202771
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