Translation and processing of MHV-A59 virion RNA in reticulocyte lysates and infected cells

M Denison; S Perlman

doi:10.1007/978-1-4684-1280-2_18

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Translation and processing of MHV-A59 virion RNA in reticulocyte lysates and infected cells

Journal article

Open access

Peer reviewed

Translation and processing of MHV-A59 virion RNA in reticulocyte lysates and infected cells

M Denison and S Perlman

Advances in experimental medicine and biology, Vol.218, pp.155-156

1987

DOI: 10.1007/978-1-4684-1280-2_18

PMID: 2829524

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Abstract

The first event in the replication of coronavirus MHV-A59, after adsorption, penetration and uncoating, must be the translation of virion RNA into an RNA-dependent RNA polymerase. The study of RNA polymerase activity in infected cells, and the identification of the proteins responsible for that activity is difficult because the protein is synthesized in very small quantities. To circumvent that problem, we have used a cell-free translation system from rabbit reticulocytes to identify the proteins coded for by input genomic RNA. We have also confirmed the validity of this approach by identifying the major in vitro translation product in infected L-2 cells.

Rabbits

Protein Biosynthesis

Animals

RNA, Viral - genetics

RNA Processing, Post-Transcriptional

L Cells (Cell Line) - metabolism

Viral Proteins - genetics

In Vitro Techniques

Reticulocytes - metabolism

Viral Proteins - biosynthesis

Murine hepatitis virus - genetics

Details

Title: Subtitle: Translation and processing of MHV-A59 virion RNA in reticulocyte lysates and infected cells
Creators: M Denison - Department of Pediatrics, University of Iowa, Iowa City 52242
S Perlman
Resource Type: Journal article
Publication Details: Advances in experimental medicine and biology, Vol.218, pp.155-156
DOI: 10.1007/978-1-4684-1280-2_18
PMID: 2829524
NLM abbreviation: Adv Exp Med Biol
ISSN: 0065-2598
Publisher: United States
Language: English
Date published: 1987
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777476902771

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