Journal article
Translation and validation of an ageism scale for dental students in Switzerland
Journal of oral science, Vol.64(1), pp.74-79
2021
DOI: 10.2334/josnusd.21-0389
Abstract
Activation of epidermal growth factor receptor (EGFR) tyrosine kinase is associated with increased extracellular signal-regulated kinase (ERK) 1/2 signaling in the hypothalamic paraventricular nucleus (PVN), which contributes to the sympathetic excitation in heart failure (HF). Transforming growth factor (TGF)-α is a major endogenous ligand for EGFR. The present study sought to determine whether TGF-α increases in the PVN in HF and promotes the activation of EGFR to increase ERK1/2 activity. Male rats received bilateral PVN microinjections of an EGFR siRNA or a scrambled siRNA followed by an intracerebroventricular (ICV) injection of TGF-α or vehicle one week later. In rats pretreated with the scrambled siRNA, ICV TGF-α increased phosphorylated (p-) EGFR and upregulated the expression of p-ERK1/2 and mRNA levels of proinflammatory cytokines (PICs) and renin-angiotensin system (RAS) components in the PVN, when compared with the untreated age-matched control rats. These responses to ICV TGF-α were significantly attenuated in rats pretreated with the EGFR siRNA. Furthermore, bilateral PVN microinjection of a TGF-α siRNA in HF rats significantly decreased the elevated levels of TGF-α, p-EGFR, p-ERK1/2 and the mRNA expression of PICs and RAS components in the PVN, compared with the HF rats treated with a scrambled siRNA. The TGF-α siRNA-treated HF rats also exhibited lower plasma norepinephrine levels and improved peripheral manifestations of HF. These data suggest that TGF-α expression is upregulated in the PVN in HF and induces the activation of EGFR-mediated ERK1/2 signaling to augment the inflammation and RAS activity that drives sympathetic excitation in HF.
Details
- Title: Subtitle
- Translation and validation of an ageism scale for dental students in Switzerland
- Creators
- Eleni Michalopoulou - University of ZurichMichael M Bornstein - University of BaselMartin Schimmel - University of BernAnastassia Kossioni - National and Kapodistrian University of AthensNicole Kalberer - University of ZurichLeonardo Marchini - University of IowaMurali Srinivasan - University of Zurich
- Resource Type
- Journal article
- Publication Details
- Journal of oral science, Vol.64(1), pp.74-79
- DOI
- 10.2334/josnusd.21-0389
- ISSN
- 1343-4934
- eISSN
- 1880-4926
- Language
- English
- Date published
- 2021
- Academic Unit
- Preventive and Community Dentistry
- Record Identifier
- 9984204088402771
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