Journal article
Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq
Molecular brain, Vol.13(1), pp.165-165
12/01/2020
DOI: 10.1186/s13041-020-00702-5
PMCID: PMC7713217
PMID: 33272296
Abstract
Sleep deprivation is a global health problem adversely affecting health as well as causing decrements in learning and performance. Sleep deprivation induces significant changes in gene transcription in many brain regions, with the hippocampus particularly susceptible to acute sleep deprivation. However, less is known about the impacts of sleep deprivation on post-transcriptional gene regulation. To identify the effects of sleep deprivation on the translatome, we took advantage of the RiboTag mouse line to express HA-labeled Rpl22 in CaMKIIα neurons to selectively isolate and sequence mRNA transcripts associated with ribosomes in excitatory neurons. We found 198 differentially expressed genes in the ribosome-associated mRNA subset after sleep deprivation. In comparison with previously published data on gene expression in the hippocampus after sleep deprivation, we found that the subset of genes affected by sleep deprivation was considerably different in the translatome compared with the transcriptome, with only 49 genes regulated similarly. Interestingly, we found 478 genes differentially regulated by sleep deprivation in the transcriptome that were not significantly regulated in the translatome of excitatory neurons. Conversely, there were 149 genes differentially regulated by sleep deprivation in the translatome but not in the whole transcriptome. Pathway analysis revealed differences in the biological functions of genes exclusively regulated in the transcriptome or translatome, with protein deacetylase activity and small GTPase binding regulated in the transcriptome and unfolded protein binding, kinase inhibitor activity, neurotransmitter receptors and circadian rhythms regulated in the translatome. These results indicate that sleep deprivation induces significant changes affecting the pool of actively translated mRNAs.
Details
- Title: Subtitle
- Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq
- Creators
- Lisa C Lyons - Iowa City, IA USASnehajyoti Chatterjee - Iowa City, IA USAYann Vanrobaeys - Iowa City, IA USAMarie E Gaine - Iowa City, IA USATed Abel - Iowa City, IA USA
- Resource Type
- Journal article
- Publication Details
- Molecular brain, Vol.13(1), pp.165-165
- Publisher
- BioMed Central; London
- DOI
- 10.1186/s13041-020-00702-5
- PMID
- 33272296
- PMCID
- PMC7713217
- ISSN
- 1756-6606
- eISSN
- 1756-6606
- Grant note
- R01 AG062398 / ;
- Language
- English
- Date published
- 12/01/2020
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984065368702771
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