Journal article
Translocation and activation of protein kinase C by the plasma cell tumor-promoting alkane pristane
Cancer research (Chicago, Ill.), Vol.55(3), pp.518-524
1995
PMID: 7834620
Abstract
Pristane (2,6,10,14-tetramethylpentadecane) is a C19-isoalkane that promotes the development of plasmacytomas in genetically susceptible BALB/c mice. Similarities between the effects of pristane and protein kinase C (PKC)-activating phorbol esters suggested that the tumor promoting activity of pristane might involve the activation of PKC. Here we show that up to 5 mol% of pristane can be homogeneously incorporated into phosphatidylcholine/phosphatidylserine bilayers. Membrane-incorporated pristane partially activated PKC and increased phorbol ester binding to the bilayer by more than 50%. Pristane (50 microM) delivered as an inclusion complex with beta-cyclodextrin to promyelocytic HL-60 leukemia cells induced a partial long-term translocation of PKC to the cell membrane. This was accompanied by differentiation of HL-60 cells into macrophage-like cells. It is concluded that activation of PKC may comprise an important aspect of the tumor promoting potential of pristane.
Details
- Title: Subtitle
- Translocation and activation of protein kinase C by the plasma cell tumor-promoting alkane pristane
- Creators
- S JANZ - NIH, national cancer inst., lab. genetics, Bethesda MD 20892, United StatesK GAWRISCH - NIH, national cancer inst., lab. genetics, Bethesda MD 20892, United StatesD. S LESTER - NIH, national cancer inst., lab. genetics, Bethesda MD 20892, United States
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.55(3), pp.518-524
- Publisher
- American Association for Cancer Research
- PMID
- 7834620
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 1995
- Academic Unit
- Pathology
- Record Identifier
- 9984083806502771
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