Journal article
Translocation remodeling in the primary BALB/c plasmacytoma TEPC 3610
Genes chromosomes & cancer, Vol.30(3), pp.283-291
03/2001
DOI: 10.1002/1098-2264(2000)9999:9999<::AID-GCC1094>3.0.CO;2-I
PMID: 11170286
Abstract
Myc-activating chromosomal 12;15 translocations, the hallmark mutations of inflammation-induced BALB/c plasmacytomas, have recently been shown to undergo remodeling by isotype switch-like genetic recombinations that remove approximately 180 kb of immunoglobulin heavy-chain sequence in the vicinity of the rearranged, expressed Myc gene. Here we combine cytogenetic data on the 12;15 translocation (SKY and FISH) with the molecular analysis of key junction sites (long-range PCR followed by DNA sequencing) to demonstrate that translocation remodeling occurred as an infrequent, stepwise, and disomic tumor progression event in the tetraploid, fully transformed, and transplantable plasmacytoma TEPC 3610. This result was used, in conjunction with previously obtained molecular data on five other primary plasmacytomas, to devise a hypothesis that predicts that the selective pressure to undergo translocation remodeling may be predetermined by the location of the break site in Myc. The pressure may be low if the break occurs 5' of the normal promoter region of Myc, but it may be considerably stronger if the break occurs 3' of the Myc promoter.
Details
- Title: Subtitle
- Translocation remodeling in the primary BALB/c plasmacytoma TEPC 3610
- Creators
- Alexander L Kovalchuk - Laboratory of Genetics, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MarylandArif Esa - Institute of Applied Physics, University of Heidelberg, Heidelberg, GermanyAllen E Coleman - Laboratory of Genetics, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MarylandSung S Park - Laboratory of Genetics, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MarylandThomas Ried - Genetics Department, Division of Clinical Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MarylandChristoph C Cremer - Institute of Applied Physics, University of Heidelberg, Heidelberg, GermanySiegfried Janz - Laboratory of Genetics, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland
- Resource Type
- Journal article
- Publication Details
- Genes chromosomes & cancer, Vol.30(3), pp.283-291
- Publisher
- John Wiley & Sons, Inc
- DOI
- 10.1002/1098-2264(2000)9999:9999<::AID-GCC1094>3.0.CO;2-I
- PMID
- 11170286
- ISSN
- 1045-2257
- eISSN
- 1098-2264
- Number of pages
- 9
- Language
- English
- Date published
- 03/2001
- Academic Unit
- Pathology
- Record Identifier
- 9984083208602771
Metrics
18 Record Views