Transmissibility of adenovirus-induced adiposity in a chicken model

N. V Dhurandhar; B. A Israel; J. M Kolesar; G Mayhew; M. E Cook; R. L Atkinson

doi:10.1038/sj.ijo.0801668

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Transmissibility of adenovirus-induced adiposity in a chicken model

Journal article

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Peer reviewed

Transmissibility of adenovirus-induced adiposity in a chicken model

N. V Dhurandhar, B. A Israel, J. M Kolesar, G Mayhew, M. E Cook and R. L Atkinson

International journal of obesity, Vol.25(7), pp.990-996

07/01/2001

DOI: 10.1038/sj.ijo.0801668

PMID: 11443497

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Abstract

BACKGROUND: We previously reported that human adenovirus Ad-36 induces adiposity and paradoxically lower levels of serum cholesterol (CHOL) and triglycerides (TG) in animals. OBJECTIVE: To evaluate the transmissibility of Ad-36 and Ad-36 induced adiposity using a chicken model. DESIGN: Experiment 1—four chickens were housed (two per cage) and one from each cage was inoculated with Ad-36. Duration of presence of Ad-36 DNA in the blood of all chickens was monitored. Experiment 2—two groups of chickens were intranasally inoculated with Ad-36 (infected donors, I-D) or media (control donors, C-D). Blood drawn 36 h later from I-D and C-D groups was inoculated into wing veins of recipient chickens (infected receivers, I-R, and control receivers, C-R, respectively). On sacrifice, 5 weeks post-inoculation, blood was drawn, body weight noted and visceral fat was separated and weighed. RESULTS: Experiment 1—Ad-36 DNA appeared in the blood of the inoculated chickens and that of uninoculated chickens (cage mates) within 12 h of inoculation and the viral DNA persisted up to 25 days in the blood. Experiment 2—compared with C-D, visceral and total body fat were significantly greater and CHOL significantly lower for the I-D and I-R. TG were significantly lower for the I-D. Ad-36 was isolated from 12 out of 16 blood samples of the I-D that were used for inoculating I-R chickens. Ad-36 DNA was present in the blood and the adipose tissue of the I-D and I-R but not in the skeletal muscles of animals selected randomly for testing. CONCLUSION: As seen in experiment 1, Ad-36 infection can be transmitted horizontally from an infected chicken to another chicken sharing the cage. Additionally, experiment 2 demonstrated blood-borne transmission of Ad-36-induced adiposity in chickens. Transmissibility of Ad-36-induced adiposity in chicken model raises serious concerns about such a possibility in humans that needs further investigation.

Obesity

Biological and medical sciences

Medical sciences

Metabolic diseases

Details

Title: Subtitle: Transmissibility of adenovirus-induced adiposity in a chicken model
Creators: N. V Dhurandhar - Wayne State University
B. A Israel - University of Wisconsin–Madison
J. M Kolesar - University of Wisconsin–Madison
G Mayhew - University of Wisconsin–Madison
M. E Cook - University of Wisconsin–Madison
R. L Atkinson - University of Wisconsin–Madison
Resource Type: Journal article
Publication Details: International journal of obesity, Vol.25(7), pp.990-996
DOI: 10.1038/sj.ijo.0801668
PMID: 11443497
NLM abbreviation: Int J Obes (Lond)
ISSN: 0307-0565
eISSN: 1476-5497
Publisher: Nature Publishing
Language: English
Date published: 07/01/2001
Academic Unit: Pharmacy; Pharmaceutical Sciences and Experimental Therapeutics
Record Identifier: 9984695786402771

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