Transplantation of iPSC-TM stimulates division of trabecular meshwork cells in human eyes

Wei Zhu; Cheyanne R Godwin; Lin Cheng; Todd E Scheetz; Markus H Kuehn

doi:10.1038/s41598-020-59941-0

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Transplantation of iPSC-TM stimulates division of trabecular meshwork cells in human eyes

Journal article

Open access

Peer reviewed

Transplantation of iPSC-TM stimulates division of trabecular meshwork cells in human eyes

Wei Zhu, Cheyanne R Godwin, Lin Cheng, Todd E Scheetz and Markus H Kuehn

Scientific reports, Vol.10(1), pp.2905-2905

02/19/2020

DOI: 10.1038/s41598-020-59941-0

PMCID: PMC7031365

PMID: 32076077

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https://doi.org/10.1038/s41598-020-59941-0View

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Abstract

The trabecular meshwork's (TM) physiological role is to maintain normal intraocular pressure by regulating aqueous humor outflow. With age, and particularly in eyes with primary open angle glaucoma, the number of cells residing within the TM is markedly decreased and the function of the tissue is compromised. Here we evaluate if transplantation of induced pluripotent stem cell derived TM like cells (iPSC-TM) restores TM cellularity and function in human eyes obtained from older human donors. Human iPSC were differentiated into iPSC-TM and compared to primary TM cells by RNAseq. iPSC-TM were then injected into the anterior segments of human eyes maintained in perfusion culture. Seven and 14 days eyes after injection eyes that received iPSC-TM contained significantly more cells in the TM. Fewer than 1% of all cells appeared to be iPSC-TM, but significantly more cells in these eyes were immunopositive for Ki 67 and incorporated BrdU. Our study demonstrates that transplantation iPSC-TM stimulates proliferation of endogenous TM cells in perfusion cultured human eyes from aged donors. These data, in concert with our previous findings in animal models, suggest that functional regeneration of the TM may be possible in human eyes with primary open angle glaucoma.

Cell Differentiation

Aged

Biomarkers - metabolism

Cell Division

Cell Proliferation

Cell Shape

Cells, Cultured

Female

Humans

Induced Pluripotent Stem Cells - cytology

Induced Pluripotent Stem Cells - transplantation

Male

Principal Component Analysis

Trabecular Meshwork - cytology

Details

Title: Subtitle: Transplantation of iPSC-TM stimulates division of trabecular meshwork cells in human eyes
Creators: Wei Zhu - Qingdao University
Cheyanne R Godwin - University of Iowa
Lin Cheng - University of Iowa
Todd E Scheetz - University of Iowa
Markus H Kuehn - University of Iowa
Resource Type: Journal article
Publication Details: Scientific reports, Vol.10(1), pp.2905-2905
DOI: 10.1038/s41598-020-59941-0
PMID: 32076077
PMCID: PMC7031365
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Grant note: I01 RX002860 / RRD VA I01 RX001163 / RRD VA P30 EY025580 / NEI NIH HHS I50 RX003002 / RRD VA
Language: English
Date published: 02/19/2020
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Ophthalmology and Visual Sciences
Record Identifier: 9984182759002771

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