Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes

D A Gordenin; A L Malkova; A Peterzen; V N Kulikov; Y I Pavlov; E Perkins; M A Resnick

doi:10.1073/pnas.89.9.3785

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Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes

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Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes

D A Gordenin, A L Malkova, A Peterzen, V N Kulikov, Y I Pavlov, E Perkins and M A Resnick

Proceedings of the National Academy of Sciences - PNAS, Vol.89(9), pp.3785-3789

05/01/1992

DOI: 10.1073/pnas.89.9.3785

PMCID: PMC525575

PMID: 1315039

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Abstract

Interaction between short repeats may be a source of genomic rearrangements and deletions. We investigated possible interactions between short (9 base pairs) direct repeats in yeast by using our previously described system for analyzing bacterial transposon Tn5 excision in yeast. Mutations of either POL3 or POL1, the proposed structural genes for polymerases delta and alpha, respectively, yield high levels of excision at semipermissive temperatures. pol2 (corresponding to polymerase epsilon) and pol2 pol3 double mutants do not exhibit enhanced excision. A majority of excision events involve direct repeats and are precise; the remaining imprecise excisions occur within or in the vicinity of the repeats. The three DNA repair pathways identified by rad1, rad6 and rad18, rad50 and rad52 mutations were examined for their possible role in Tn5 excision; no enhancement was observed in mutants. However, the pol3-stimulated Tn5 excision was reduced in rad52 and rad50 mutants. This suggests the potential for interaction between the systems for DNA double-strand break/recombinational repair and DNA synthesis. Based on the suggestion of Morrison et al. [Morrison, A., Araki, H., Clark, A. B., Hamatake, R. H. & Sugino, A. (1990) Cell 62, 1143-1151] that polymerases delta and alpha are responsible for lagging-strand synthesis and that polymerase epsilon is responsible for leading-strand synthesis, we suggest that Tn5 excision is stimulated under conditions of altered lagging-strand synthesis, possibly due to extended opportunities for single-strand interactions between the inverted insertion sequence I550 repeats of Tn5.

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Title: Subtitle: Transposon Tn5 excision in yeast: influence of DNA polymerases alpha, delta, and epsilon and repair genes
Creators: D A Gordenin - Department of Genetics, Leningrad University, U.S.S.R
A L Malkova - Department of Genetics, Leningrad University, U.S.S.R
A Peterzen - Department of Genetics, Leningrad University, U.S.S.R
V N Kulikov - Department of Genetics, Leningrad University, U.S.S.R
Y I Pavlov - Department of Genetics, Leningrad University, U.S.S.R
E Perkins - Department of Genetics, Leningrad University, U.S.S.R
M A Resnick - Department of Genetics, Leningrad University, U.S.S.R
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.89(9), pp.3785-3789
DOI: 10.1073/pnas.89.9.3785
PMID: 1315039
PMCID: PMC525575
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 05/01/1992
Academic Unit: Biology
Record Identifier: 9984217425502771

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