Journal article
Treatment of established asthma in a murine model using CpG oligodeoxynucleotides
American journal of physiology. Lung cellular and molecular physiology, Vol.283(1), pp.L170-L179
07/01/2002
DOI: 10.1152/ajplung.00402.2001
PMID: 12060574
Abstract
Allergen immunotherapy is an effective but underutilized treatment for atopic asthma. We have previously demonstrated that CpG oligodeoxynucleotides (CpG ODN) can prevent the development of a murine model of asthma. In the current study, we evaluated the role of CpG ODN in the treatment of established eosinophilic airway inflammation and bronchial hyperreactivity in a murine model of asthma. In this model, mice with established ovalbumin (OVA)-induced airway disease were given a course of immunotherapy (using low doses of OVA) in the presence or absence of CpG ODN. All mice then were rechallenged with experimental allergen. Untreated mice developed marked airway eosinophilia and bronchial hyperresponsiveness, which were significantly reduced by treatment with OVA and CpG. CpG ODN leads to induction of antigen-induced Th1 cytokine responses; successful therapy was associated with induction of the chemokines interferon-γ-inducible protein-10 and RANTES and suppression of eotaxin. Unlike previous studies, these data demonstrate that the combination of CpG ODN and allergen can effectively reverse established atopic eosinophilic airway disease, at least partially through redirecting a Th2 to a Th1 response.
Details
- Title: Subtitle
- Treatment of established asthma in a murine model using CpG oligodeoxynucleotides
- Creators
- Joel N Kline - Department of Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Kunihiko Kitagaki - Department of Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Thomas R Businga - Department of Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242Vipul V Jain - Department of Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.283(1), pp.L170-L179
- DOI
- 10.1152/ajplung.00402.2001
- PMID
- 12060574
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Language
- English
- Date published
- 07/01/2002
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Occupational and Environmental Health; Pathology; Internal Medicine
- Record Identifier
- 9984094626102771
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