Journal article
Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae
Bio-protocol, Vol.12(2), e4289
01/20/2022
DOI: 10.21769/BioProtoc.4289
PMCID: PMC8799664
PMID: 35127979
Abstract
Larval zebrafish have been established as an excellent model for examining vertebrate biology, with many researchers using the system for neuroscience. Controlling a fast escape response of the fish, the Mauthner cells and their associated network are an attractive model, given their experimental accessibility and fast development, driving ethologically relevant behavior in the first five days of development. Here, we describe methods for immunostaining electrical and chemical synapse proteins at 3-7 days post fertilization (dpf) in zebrafish using tricholoracetic acid fixation. The methods presented are ideally suited to easily visualize neural circuits and synapses within the fish.
Details
- Title: Subtitle
- Trichloroacetic Acid Fixation and Antibody Staining of Zebrafish Larvae
- Creators
- E Anne Martin - University of OregonSundas Ijaz - Albert Einstein College of MedicineAlberto Pereda - Albert Einstein College of MedicineAdam Miller - University of Oregon
- Resource Type
- Journal article
- Publication Details
- Bio-protocol, Vol.12(2), e4289
- DOI
- 10.21769/BioProtoc.4289
- PMID
- 35127979
- PMCID
- PMC8799664
- NLM abbreviation
- Bio Protoc
- ISSN
- 2331-8325
- eISSN
- 2331-8325
- Publisher
- Bio-Protocol
- Number of pages
- 6
- Grant note
- RF1MH120016 / National Institute of Mental Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) R01DC011099 / National Institute on Deafness and Other Communication Disorders; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Deafness & Other Communication Disorders (NIDCD) R21NS085772; R01NS105758 / National Institute of Neurological Disorders and Stroke; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) F32HD102182 / Eunice Kennedy Shriver National Institute of Child Health and Human Development; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Language
- English
- Date published
- 01/20/2022
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984937805202771
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