Trimester-specific plasma exosome microRNA expression profiles in preeclampsia

Eric Devor; Donna Santillan; Sabrina Scroggins; Akshaya Warrier; Mark Santillan

doi:10.1080/14767058.2019.1569614

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Trimester-specific plasma exosome microRNA expression profiles in preeclampsia

Journal article

Open access

Trimester-specific plasma exosome microRNA expression profiles in preeclampsia

Eric Devor, Donna Santillan, Sabrina Scroggins, Akshaya Warrier and Mark Santillan

The journal of maternal-fetal & neonatal medicine, Vol.33(18), pp.3116-3124

01/30/2019

DOI: 10.1080/14767058.2019.1569614

PMID: 30700172

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https://www.ncbi.nlm.nih.gov/pmc/articles/7328825View

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Abstract

To identify microRNAs (miRNAs) differentially expressed in plasma exosomes collected in women diagnosed with preeclampsia compared with women with uncomplicated pregnancies. Exosomes were purified from plasma samples obtained at each trimester from four women subsequently diagnosed with preeclampsia and from five matched healthy controls. RNA was purified from the exosomes, and expression of 368 miRNAs was profiled using A-Set TaqMan low density array (TLDA). One-third of the 368 miRNAs profiled are not expressed in exosomes. Further, those that are not expressed tend to be evolutionarily younger and have a significantly different mature sequence signature than do miRNAs that are expressed in exosomes. Among miRNAs that are expressed in exosomes, a total of eight (miR-134, miR-196b, miR-302c, miR-346, miR-376c, miR-486-3p, miR-590-5p, and miR-618) were found to display statistically significant differential expression between women who developed preeclampsia as compared with those who did not. Moreover, half of these miRNAs (miR-134, miR-376c, miR-486-3p, and miR-590-5p) displayed statistically significant differential expression in the first trimester. Not all miRNAs are expressed in exosomes. Those that tend to be evolutionarily older and have a significantly different mature sequence signature than those that are not. A few exosome-expressed miRNAs do display expression patterns in women subsequently diagnosed with preeclampsia that are significantly different than in women having an uncomplicated and, among these, several appear in the first trimester. These miRNAs are potential early markers of preeclampsia risk.

microRNA

preeclampsia

Exosome

trimester-specific

profile

Details

Title: Subtitle: Trimester-specific plasma exosome microRNA expression profiles in preeclampsia
Creators: Eric Devor - a Obstetrics and Gynecology, Roy J and Lucille A Carver College of Medicine , University of Iowa , Iowa City , IA , USA
Donna Santillan - a Obstetrics and Gynecology, Roy J and Lucille A Carver College of Medicine , University of Iowa , Iowa City , IA , USA
Sabrina Scroggins - a Obstetrics and Gynecology, Roy J and Lucille A Carver College of Medicine , University of Iowa , Iowa City , IA , USA
Akshaya Warrier - a Obstetrics and Gynecology, Roy J and Lucille A Carver College of Medicine , University of Iowa , Iowa City , IA , USA
Mark Santillan - b Obstetrics and Gynecology , University of Iowa Hospitals and Clinics , Iowa City , IA , USA
Resource Type: Journal article
Publication Details: The journal of maternal-fetal & neonatal medicine, Vol.33(18), pp.3116-3124
DOI: 10.1080/14767058.2019.1569614
PMID: 30700172
NLM abbreviation: J Matern Fetal Neonatal Med
ISSN: 1476-4954
eISSN: 1476-4954
Publisher: England
Language: English
Electronic publication date: 01/30/2019
Academic Unit: Obstetrics and Gynecology
Record Identifier: 9983930389302771

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