Triple Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission at Central GABAergic Synapses

Chong Chen; Rachel Satterfield; Samuel M Young; Peter Jonas

doi:10.1016/j.celrep.2017.10.122

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Triple Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission at Central GABAergic Synapses

Journal article

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Triple Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission at Central GABAergic Synapses

Chong Chen, Rachel Satterfield, Samuel M Young and Peter Jonas

Cell reports (Cambridge), Vol.21(8), pp.2082-2089

11/21/2017

DOI: 10.1016/j.celrep.2017.10.122

PMCID: PMC5863544

PMID: 29166601

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Abstract

Synaptotagmin 7 (Syt7) is thought to be a Ca2+ sensor that mediates asynchronous transmitter release and facilitation at synapses. However, Syt7 is strongly expressed in fast-spiking, parvalbumin-expressing GABAergic interneurons, and the output synapses of these neurons produce only minimal asynchronous release and show depression rather than facilitation. To resolve this apparent contradiction, we examined the effects of genetic elimination of Syt7 on synaptic transmission at the GABAergic basket cell (BC)-Purkinje cell (PC) synapse in cerebellum. Our results indicate that at the BC-PC synapse, Syt7 contributes to asynchronous release, pool replenishment, and facilitation. In combination, these three effects ensure efficient transmitter release during high-frequency activity and guarantee frequency independence of inhibition. Our results identify a distinct function of Syt7: ensuring the efficiency of high-frequency inhibitory synaptic transmission. [Display omitted] •Syt7 contributes to asynchronous release, facilitation, and replenishment•Syt7 conveys efficient and frequency-independent release during repetitive activity•Syt7 enables the powerful control of PC activity by single BC synaptic inputs•Syt7 has similar functions at cerebellar and hippocampal BC synapses Chen et al. demonstrate that synaptotagmin 7 at cerebellar inhibitory synapses functions as a Ca2+ sensor for asynchronous release, facilitation, and vesicle pool replenishment. This triple function of Syt7 ensures efficacy and frequency independence of inhibitory synaptic transmission.

transmitter release

Ca2+ sensor

pool replenishment

fast spiking

sustained release

endocytosis

GABAergic synapses

exocytosis

synaptotagmin

cerebellum

basket cells

Details

Title: Subtitle: Triple Function of Synaptotagmin 7 Ensures Efficiency of High-Frequency Transmission at Central GABAergic Synapses
Creators: Chong Chen - IST Austria (Institute of Science and Technology Austria), Am Campus 1, A-3400 Klosterneuburg, Austria
Rachel Satterfield - Max Planck Florida Institute for Neuroscience, Research Group Molecular Mechanisms of Synaptic Function, Jupiter, FL 33458, USA
Samuel M Young - Max Planck Florida Institute for Neuroscience, Research Group Molecular Mechanisms of Synaptic Function, Jupiter, FL 33458, USA
Peter Jonas - IST Austria (Institute of Science and Technology Austria), Am Campus 1, A-3400 Klosterneuburg, Austria
Resource Type: Journal article
Publication Details: Cell reports (Cambridge), Vol.21(8), pp.2082-2089
DOI: 10.1016/j.celrep.2017.10.122
PMID: 29166601
PMCID: PMC5863544
NLM abbreviation: Cell Rep
ISSN: 2211-1247
eISSN: 2211-1247
Publisher: Elsevier Inc
Language: English
Date published: 11/21/2017
Academic Unit: Anatomy and Cell Biology; Iowa Neuroscience Institute; Otolaryngology
Record Identifier: 9984025307402771

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