Journal article
Trypanosoma cruzi : interruption of both alleles of a gene encoding a protein containing 14-amino-acid repeats by targeted insertion of NEOr and HYGr
Experimental parasitology, Vol.81(4), pp.529-535
1995
DOI: 10.1006/expr.1995.1146
PMID: 8542994
Abstract
In Trypanosoma cruzi approximately 90% of the 121- and 176-kDa cytoskeletal proteins encoded by the two alleles of the TCR27 gene is composed of 14-amino-acid repeats. To gain insight into the function of the TCR27 proteins we replaced the corresponding regions of Q-nucleotide repeats in the two alleles with the NEO(r) and HYG(r) genes. Analyses of DNAs and RNAs from four clones resistant to both G418, a neomycin analogue, and hygromycin showed that in both cases the repetitive regions had in fact been deleted. In addition, the absence of expression of the 14-amino-acid repeats was confirmed in Western blots. In axenic cultures growth rates of the morphologically unchanged, doubly resistant organisms were not different from those of wild-type parasites. However, the doubly resistant organisms proliferated more slowly in cultured mammalian cells than did wild-type parasites. These findings indicate that the absence of the TCR27 repetitive regions is detrimental, but not fatal, to the parasites. © 1995 Academic Press, Inc.
Details
- Title: Subtitle
- Trypanosoma cruzi : interruption of both alleles of a gene encoding a protein containing 14-amino-acid repeats by targeted insertion of NEOr and HYGr
- Creators
- K Otsu - University of IowaJ. E Donelson - Univ. Iowa, dep. internal medicine, 300G EMRB, Iowa City IA 52242, United StatesL. V Kirchhoff - Univ. Iowa, dep. internal medicine, 300G EMRB, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Experimental parasitology, Vol.81(4), pp.529-535
- Publisher
- Elsevier
- DOI
- 10.1006/expr.1995.1146
- PMID
- 8542994
- ISSN
- 0014-4894
- eISSN
- 1090-2449
- Language
- English
- Date published
- 1995
- Academic Unit
- Psychiatry; Infectious Diseases; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984280836602771
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